310d

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[[Image:310d.jpg|left|200px]]
 
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==Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA==
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The line below this paragraph, containing "STRUCTURE_310d", creates the "Structure Box" on the page.
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<StructureSection load='310d' size='340' side='right'caption='[[310d]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[310d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=310D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=310D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene></td></tr>
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{{STRUCTURE_310d| PDB=310d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=310d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=310d OCA], [https://pdbe.org/310d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=310d RCSB], [https://www.ebi.ac.uk/pdbsum/310d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=310d ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF 2'-O-ME(CGCGCG)2: AN RNA DUPLEX AT 1.3 A RESOLUTION. HYDRATION PATTERN OF 2'-O-METHYLATED RNA'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Synthetic construct]]
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The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been determined using synchrotron radiation at near-atomic resolution (1.30 A), the highest resolution to date in the RNA field. The crystal structure is a half-turn A-type helix with some helical parameters deviating from canonical A-RNA, such as low base pair rise, elevated helical twist and inclination angles. In CG steps, inter-strand guanines are parallel while cytosines are not parallel. In steps GC this motif is reversed. This type of regularity is not seen in other RNA crystal structures. The structure includes 44 water molecules and two hydrated Mg2+ions one of which lies exactly on the crystallographic 2-fold axis. There are distinct patterns of hydration in the major and the minor grooves. The major groove is stabilised by water clusters consisting of fused five- and six-membered rings. Minor groove contains only a single row of water molecules; each water bridges either two self-parallel cytosines or two self-parallel guanines by a pair of hydrogen bonds. The structure provides the first view of the hydration scheme of 2'-O-methylated RNA duplex.
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[[Category: Adamiak DA]]
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[[Category: Adamiak RW]]
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==About this Structure==
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[[Category: Dauter Z]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=310D OCA].
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[[Category: Milecki J]]
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[[Category: Rypniewski WR]]
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==Reference==
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Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA., Adamiak DA, Milecki J, Popenda M, Adamiak RW, Dauter Z, Rypniewski WR, Nucleic Acids Res. 1997 Nov 15;25(22):4599-607. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9358171 9358171]
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[[Category: Adamiak, D A.]]
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[[Category: Adamiak, R W.]]
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[[Category: Dauter, Z.]]
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[[Category: Milecki, J.]]
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[[Category: Rypniewski, W R.]]
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[[Category: A-rna]]
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[[Category: Double helix]]
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[[Category: Modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:15:07 2008''
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Current revision

Crystal structure of 2'-O-Me(CGCGCG)2: an RNA duplex at 1.3 A resolution. Hydration pattern of 2'-O-methylated RNA

PDB ID 310d

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