1aj4

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(New page: 200px<br /><applet load="1aj4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aj4" /> '''STRUCTURE OF CALCIUM-SATURATED CARDIAC TROPO...)
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[[Image:1aj4.gif|left|200px]]<br /><applet load="1aj4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aj4" />
 
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'''STRUCTURE OF CALCIUM-SATURATED CARDIAC TROPONIN C, NMR, 1 STRUCTURE'''<br />
 
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==Overview==
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==STRUCTURE OF CALCIUM-SATURATED CARDIAC TROPONIN C, NMR, 1 STRUCTURE==
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The regulation of cardiac muscle contraction must differ from that of, skeletal muscles to effect different physiological and contractile, properties. Cardiac troponin C (TnC), the key regulator of cardiac muscle, contraction, possesses different functional and Ca2+-binding properties, compared with skeletal TnC and features a Ca2+-binding site I, which is, naturally inactive. The structure of cardiac TnC in the Ca2+-saturated, state has been determined by nuclear magnetic resonance spectroscopy. The, regulatory domain exists in a "closed" conformation even in the Ca2+-bound, (the "on") state, in contrast to all predicted models and differing, significantly from the calcium-induced structure observed in skeletal TnC., This structure in the Ca2+-bound state, and its subsequent interaction, with troponin I (TnI), are crucial in determining the specific regulatory, mechanism for cardiac muscle contraction. Further, it will allow for an, understanding of the action of calcium-sensitizing drugs, which bind to, cardiac TnC and are known to enhance the ability of cardiac TnC to, activate cardiac muscle contraction.
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<StructureSection load='1aj4' size='340' side='right'caption='[[1aj4]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1aj4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AJ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aj4 OCA], [https://pdbe.org/1aj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aj4 RCSB], [https://www.ebi.ac.uk/pdbsum/1aj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aj4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TNNC1_CHICK TNNC1_CHICK] Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/1aj4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aj4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The regulation of cardiac muscle contraction must differ from that of skeletal muscles to effect different physiological and contractile properties. Cardiac troponin C (TnC), the key regulator of cardiac muscle contraction, possesses different functional and Ca2+-binding properties compared with skeletal TnC and features a Ca2+-binding site I, which is naturally inactive. The structure of cardiac TnC in the Ca2+-saturated state has been determined by nuclear magnetic resonance spectroscopy. The regulatory domain exists in a "closed" conformation even in the Ca2+-bound (the "on") state, in contrast to all predicted models and differing significantly from the calcium-induced structure observed in skeletal TnC. This structure in the Ca2+-bound state, and its subsequent interaction with troponin I (TnI), are crucial in determining the specific regulatory mechanism for cardiac muscle contraction. Further, it will allow for an understanding of the action of calcium-sensitizing drugs, which bind to cardiac TnC and are known to enhance the ability of cardiac TnC to activate cardiac muscle contraction.
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==About this Structure==
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Structure of cardiac muscle troponin C unexpectedly reveals a closed regulatory domain.,Sia SK, Li MX, Spyracopoulos L, Gagne SM, Liu W, Putkey JA, Sykes BD J Biol Chem. 1997 Jul 18;272(29):18216-21. PMID:9218458<ref>PMID:9218458</ref>
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1AJ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AJ4 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of cardiac muscle troponin C unexpectedly reveals a closed regulatory domain., Sia SK, Li MX, Spyracopoulos L, Gagne SM, Liu W, Putkey JA, Sykes BD, J Biol Chem. 1997 Jul 18;272(29):18216-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9218458 9218458]
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</div>
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[[Category: Gallus gallus]]
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<div class="pdbe-citations 1aj4" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Gagne, S.M.]]
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[[Category: Li, M.X.]]
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[[Category: Liu, W.]]
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[[Category: Putkey, J.A.]]
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[[Category: Sia, S.K.]]
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[[Category: Spyracopoulos, L.]]
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[[Category: Sykes, B.D.]]
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[[Category: CA]]
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[[Category: calcium binding]]
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[[Category: cardiac]]
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[[Category: muscle protein]]
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[[Category: regulatory]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:53:47 2007''
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==See Also==
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*[[Troponin 3D structures|Troponin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Gallus gallus]]
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[[Category: Large Structures]]
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[[Category: Gagne SM]]
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[[Category: Li MX]]
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[[Category: Liu W]]
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[[Category: Putkey JA]]
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[[Category: Sia SK]]
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[[Category: Spyracopoulos L]]
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[[Category: Sykes BD]]

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STRUCTURE OF CALCIUM-SATURATED CARDIAC TROPONIN C, NMR, 1 STRUCTURE

PDB ID 1aj4

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