3b4r

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[[Image:3b4r.jpg|left|200px]]
 
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==Site-2 Protease from Methanocaldococcus jannaschii==
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The line below this paragraph, containing "STRUCTURE_3b4r", creates the "Structure Box" on the page.
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<StructureSection load='3b4r' size='340' side='right'caption='[[3b4r]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3b4r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. The August 2011 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Rhomboid Protease GlpG'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2011_8 10.2210/rcsb_pdb/mom_2011_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B4R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3b4r| PDB=3b4r | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b4r OCA], [https://pdbe.org/3b4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b4r RCSB], [https://www.ebi.ac.uk/pdbsum/3b4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b4r ProSAT]</span></td></tr>
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</table>
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'''Site-2 Protease from Methanocaldococcus jannaschii'''
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== Function ==
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[https://www.uniprot.org/uniprot/Y392_METJA Y392_METJA] A site-2 regulated intramembrane protease (S2P), its endogenous substrate is unknown. Regulated intramembrane proteolysis (RIP) occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. A membrane-spanning regulatory substrate protein is first cut extracytoplasmically (site-1 protease, S1P), then within the membrane itself (site-2 protease, S2P, this enzyme), while cytoplasmic proteases finish degrading the regulatory protein, liberating the effector protein. Possible signals, S1P and substrates are unknown in this organism.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Regulated intramembrane proteolysis by members of the site-2 protease (S2P) family is an important signaling mechanism conserved from bacteria to humans. Here we report the crystal structure of the transmembrane core domain of an S2P metalloprotease from Methanocaldococcus jannaschii. The protease consists of six transmembrane segments, with the catalytic zinc atom coordinated by two histidine residues and one aspartate residue approximately 14 angstroms into the lipid membrane surface. The protease exhibits two distinct conformations in the crystals. In the closed conformation, the active site is surrounded by transmembrane helices and is impermeable to substrate peptide; water molecules gain access to zinc through a polar, central channel that opens to the cytosolic side. In the open conformation, transmembrane helices alpha1 and alpha6 separate from each other by 10 to 12 angstroms, exposing the active site to substrate entry. The structure reveals how zinc embedded in an integral membrane protein can catalyze peptide cleavage.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b4/3b4r_consurf.spt"</scriptWhenChecked>
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3B4R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B4R OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of a site-2 protease family intramembrane metalloprotease., Feng L, Yan H, Wu Z, Yan N, Wang Z, Jeffrey PD, Shi Y, Science. 2007 Dec 7;318(5856):1608-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18063795 18063795]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b4r ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Single protein]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Feng, L.]]
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[[Category: Rhomboid Protease GlpG]]
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[[Category: Jeffrey, P D.]]
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[[Category: Feng L]]
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[[Category: Shi, Y.]]
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[[Category: Jeffrey PD]]
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[[Category: Wang, Z.]]
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[[Category: Shi Y]]
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[[Category: Wu, Z.]]
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[[Category: Wang Z]]
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[[Category: Yan, H.]]
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[[Category: Wu Z]]
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[[Category: Yan, N.]]
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[[Category: Yan H]]
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[[Category: Cbs domain]]
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[[Category: Yan N]]
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[[Category: Hydrolase]]
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[[Category: Intramembrane protease]]
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[[Category: Metal-binding]]
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[[Category: Metalloprotease]]
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[[Category: Transmembrane]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:23:22 2008''
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Current revision

Site-2 Protease from Methanocaldococcus jannaschii

PDB ID 3b4r

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