3b8a

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[[Image:3b8a.jpg|left|200px]]
 
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==Crystal structure of yeast hexokinase PI in complex with glucose==
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The line below this paragraph, containing "STRUCTURE_3b8a", creates the "Structure Box" on the page.
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<StructureSection load='3b8a' size='340' side='right'caption='[[3b8a]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3b8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B8A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3b8a| PDB=3b8a | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b8a OCA], [https://pdbe.org/3b8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b8a RCSB], [https://www.ebi.ac.uk/pdbsum/3b8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b8a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HXKA_YEAST HXKA_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/3b8a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b8a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hexokinase is the first enzyme in the glycolytic pathway that catalyzes the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. Two yeast hexokinase isozymes are known, namely PI and PII. Here we redetermined the crystal structure of yeast hexokinase PI from Saccharomyces cerevisiae as a complex with its substrate, glucose, and refined it at 2.95 A resolution. Comparison of the holo-PI yeast hexokinase and apo-hexokinase structures shows in detail the rigid body domain closure and specific loop movements as glucose binds and sheds more light on structural basis of the "induced fit" mechanism of reaction in the HK enzymatic action. We also performed statistical coupling analysis of the hexokinase family, which reveals two co-evolved continuous clusters of amino acid residues and shows that the evolutionary coupled amino acid residues are mostly confined to the active site and the hinge region, further supporting the importance of these parts of the protein for the enzymatic catalysis. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
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'''Crystal structure of yeast hexokinase PI in complex with glucose'''
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Crystal structure of yeast hexokinase PI in complex with glucose: A classical "induced fit" example revised.,Kuser P, Cupri F, Bleicher L, Polikarpov I Proteins. 2008 Feb 7;. PMID:18260108<ref>PMID:18260108</ref>
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==Overview==
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Hexokinase is the first enzyme in the glycolytic pathway that catalyzes the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. Two yeast hexokinase isozymes are known, namely PI and PII. Here we redetermined the crystal structure of yeast hexokinase PI from Saccharomyces cerevisiae as a complex with its substrate, glucose, and refined it at 2.95 A resolution. Comparison of the holo-PI yeast hexokinase and apo-hexokinase structures shows in detail the rigid body domain closure and specific loop movements as glucose binds and sheds more light on structural basis of the "induced fit" mechanism of reaction in the HK enzymatic action. We also performed statistical coupling analysis of the hexokinase family, which reveals two co-evolved continuous clusters of amino acid residues and shows that the evolutionary coupled amino acid residues are mostly confined to the active site and the hinge region, further supporting the importance of these parts of the protein for the enzymatic catalysis. Proteins 2008. (c) 2008 Wiley-Liss, Inc.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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3B8A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B8A OCA].
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</div>
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<div class="pdbe-citations 3b8a" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Crystal structure of yeast hexokinase PI in complex with glucose: A classical "induced fit" example revised., Kuser P, Cupri F, Bleicher L, Polikarpov I, Proteins. 2008 Feb 7;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18260108 18260108]
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*[[Hexokinase 3D structures|Hexokinase 3D structures]]
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[[Category: Hexokinase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bleicher L]]
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[[Category: Bleicher, L.]]
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[[Category: Cupri F]]
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[[Category: Cupri, F.]]
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[[Category: Kuser P]]
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[[Category: Kuser, P.]]
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[[Category: Polikarpov I]]
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[[Category: Polikarpov, I.]]
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[[Category: Allosteric enzyme]]
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[[Category: Atp-binding]]
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[[Category: Glycolysis]]
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[[Category: Induced fit]]
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[[Category: Kinase]]
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[[Category: Nucleotide-binding]]
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[[Category: Phosphorylation]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:30:30 2008''
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Current revision

Crystal structure of yeast hexokinase PI in complex with glucose

PDB ID 3b8a

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