1al8

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(New page: 200px<br /><applet load="1al8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1al8, resolution 2.2&Aring;" /> '''THREE-DIMENSIONAL STR...)
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[[Image:1al8.gif|left|200px]]<br /><applet load="1al8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1al8, resolution 2.2&Aring;" />
 
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'''THREE-DIMENSIONAL STRUCTURE OF GLYCOLATE OXIDASE WITH BOUND ACTIVE-SITE INHIBITORS'''<br />
 
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==Overview==
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==THREE-DIMENSIONAL STRUCTURE OF GLYCOLATE OXIDASE WITH BOUND ACTIVE-SITE INHIBITORS==
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A key step in plant photorespiration, the oxidation of glycolate to, glyoxylate, is carried out by the peroxisomal flavoprotein glycolate, oxidase (EC 1.1.3.15). The three-dimensional structure of this alpha/beta, barrel protein has been refined to 2 A resolution (Lindqvist Y. 1989. J, Mol Biol 209:151-166). FMN dependent glycolate oxidase is a member of the, family of alpha-hydroxy acid oxidases. Here we describe the, crystallization and structure determination of two inhibitor complexes of, the enzyme, TKP (3-Decyl-2,5-dioxo-4-hydroxy-3-pyrroline) and TACA, (4-Carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole). The structure of the, TACA complex has been refined to 2.6 A resolution and the TKP complex, solved with molecular replacement, to 2.2 A resolution. The Rfree for the, TACA and TKP complexes are 24.2 and 25.1%, respectively. The overall, structures are very similar to the unliganded holoenzyme, but a closer, examination of the active site reveals differences in the positioning of, the flavin isoalloxazine ring and a displaced flexible loop in the TKP, complex. The two inhibitors differ in binding mode and hydrophobic, interactions, and these differences are reflected by the very different Ki, values for the inhibitors, 16 nM for TACA and 4.8 microM for TKP., Implications of the structures of these enzyme-inhibitor complexes for the, model for substrate binding and catalysis proposed from the holo-enzyme, structure are discussed.
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<StructureSection load='1al8' size='340' side='right'caption='[[1al8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1al8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AL8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHP:3-DECYL-2,5-DIOXO-4-HYDROXY-3-PYRROLINE'>DHP</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1al8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1al8 OCA], [https://pdbe.org/1al8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1al8 RCSB], [https://www.ebi.ac.uk/pdbsum/1al8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1al8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GOX_SPIOL GOX_SPIOL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/al/1al8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1al8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A key step in plant photorespiration, the oxidation of glycolate to glyoxylate, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). The three-dimensional structure of this alpha/beta barrel protein has been refined to 2 A resolution (Lindqvist Y. 1989. J Mol Biol 209:151-166). FMN dependent glycolate oxidase is a member of the family of alpha-hydroxy acid oxidases. Here we describe the crystallization and structure determination of two inhibitor complexes of the enzyme, TKP (3-Decyl-2,5-dioxo-4-hydroxy-3-pyrroline) and TACA (4-Carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole). The structure of the TACA complex has been refined to 2.6 A resolution and the TKP complex, solved with molecular replacement, to 2.2 A resolution. The Rfree for the TACA and TKP complexes are 24.2 and 25.1%, respectively. The overall structures are very similar to the unliganded holoenzyme, but a closer examination of the active site reveals differences in the positioning of the flavin isoalloxazine ring and a displaced flexible loop in the TKP complex. The two inhibitors differ in binding mode and hydrophobic interactions, and these differences are reflected by the very different Ki values for the inhibitors, 16 nM for TACA and 4.8 microM for TKP. Implications of the structures of these enzyme-inhibitor complexes for the model for substrate binding and catalysis proposed from the holo-enzyme structure are discussed.
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==About this Structure==
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Three-dimensional structures of glycolate oxidase with bound active-site inhibitors.,Stenberg K, Lindqvist Y Protein Sci. 1997 May;6(5):1009-15. PMID:9144771<ref>PMID:9144771</ref>
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1AL8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea] with FMN and DHP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/(S)-2-hydroxy-acid_oxidase (S)-2-hydroxy-acid oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.15 1.1.3.15] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AL8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Three-dimensional structures of glycolate oxidase with bound active-site inhibitors., Stenberg K, Lindqvist Y, Protein Sci. 1997 May;6(5):1009-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9144771 9144771]
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</div>
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[[Category: (S)-2-hydroxy-acid oxidase]]
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<div class="pdbe-citations 1al8" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Spinacia oleracea]]
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[[Category: Lindqvist, Y.]]
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[[Category: Stenberg, K.]]
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[[Category: DHP]]
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[[Category: FMN]]
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[[Category: drug design]]
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[[Category: flavoprotein]]
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[[Category: inhibitor binding]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:56:27 2007''
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==See Also==
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*[[Glycolate oxidase 3D structures|Glycolate oxidase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Spinacia oleracea]]
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[[Category: Lindqvist Y]]
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[[Category: Stenberg K]]

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THREE-DIMENSIONAL STRUCTURE OF GLYCOLATE OXIDASE WITH BOUND ACTIVE-SITE INHIBITORS

PDB ID 1al8

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