1am2

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(New page: 200px<br /><applet load="1am2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1am2, resolution 2.2&Aring;" /> '''GYRA INTEIN FROM MYCO...)
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[[Image:1am2.gif|left|200px]]<br /><applet load="1am2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1am2, resolution 2.2&Aring;" />
 
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'''GYRA INTEIN FROM MYCOBACTERIUM XENOPI'''<br />
 
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==Overview==
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==GYRA INTEIN FROM MYCOBACTERIUM XENOPI==
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Several genes from prokaryotes and lower eukaryotes have been found to, contain an in-frame open reading frame, which encodes for an internal, protein (intein). Post-translationally, the internal polypeptide, auto-splices and ligates the external sequences to yield a functional, external protein (extein) and an intein. Most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain, that enables them to maintain their presence by a homing mechanism. We, report here the crystal structure of an intein found in the gyrase A, subunit from Mycobacterium xenopi at 2.2 A resolution. The structure, contains an unusual beta-fold with the catalytic splice junctions at the, ends of two adjacent antiparallel beta-strands. The arrangement of the, active site residues Ser 1, Thr 72, His 75, His 197, and Asn 198 is, consistent with a four-step mechanism for the cleavage-ligation reaction., Using site-directed mutagenesis, the N-terminal cysteine, proposed as the, nucleophile in the first step of the splicing reaction, was changed to a, Ser 1 and Ala 0, thus capturing the intein in a pre-spliced state.
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<StructureSection load='1am2' size='340' side='right'caption='[[1am2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1am2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_xenopi Mycobacterium xenopi]. The November 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Inteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_11 10.2210/rcsb_pdb/mom_2010_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AM2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AM2 FirstGlance]. <br>
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1AM2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_xenopi Mycobacterium xenopi]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AM2 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1am2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1am2 OCA], [https://pdbe.org/1am2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1am2 RCSB], [https://www.ebi.ac.uk/pdbsum/1am2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1am2 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing., Klabunde T, Sharma S, Telenti A, Jacobs WR Jr, Sacchettini JC, Nat Struct Biol. 1998 Jan;5(1):31-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9437427 9437427]
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== Function ==
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[https://www.uniprot.org/uniprot/GYRA_MYCXE GYRA_MYCXE] DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/am/1am2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1am2 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Inteins]]
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[[Category: Large Structures]]
[[Category: Mycobacterium xenopi]]
[[Category: Mycobacterium xenopi]]
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[[Category: Single protein]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Klabunde, T.]]
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[[Category: Klabunde T]]
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[[Category: Sacchettini, J.C.]]
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[[Category: Sacchettini JC]]
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[[Category: Sharma, S.]]
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[[Category: Sharma S]]
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[[Category: intein]]
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[[Category: protein splicing]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:57:18 2007''
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Current revision

GYRA INTEIN FROM MYCOBACTERIUM XENOPI

PDB ID 1am2

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