3beo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:28, 21 February 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3beo.jpg|left|200px]]
 
-
<!--
+
==A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases==
-
The line below this paragraph, containing "STRUCTURE_3beo", creates the "Structure Box" on the page.
+
<StructureSection load='3beo' size='340' side='right'caption='[[3beo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3beo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BEO FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UD1:URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE'>UD1</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-
{{STRUCTURE_3beo| PDB=3beo | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3beo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3beo OCA], [https://pdbe.org/3beo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3beo RCSB], [https://www.ebi.ac.uk/pdbsum/3beo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3beo ProSAT]</span></td></tr>
-
 
+
</table>
-
'''A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/A0A348ACF3_BACAN A0A348ACF3_BACAN]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The non-hydrolysing bacterial UDP-N-acetylglucosamine 2-epimerase (UDP-GlcNAc 2-epimerase) catalyses the conversion of UDP-GlcNAc into UDP-N-acetylmannosamine, an intermediate in the biosynthesis of several cell-surface polysaccharides. This enzyme is allosterically regulated by its substrate UDP-GlcNAc. The structure of the ternary complex between the Bacillus anthracis UDP-GlcNAc 2-epimerase, its substrate UDP-GlcNAc and the reaction intermediate UDP, showed direct interactions between UDP and its substrate, and between the complex and highly conserved enzyme residues, identifying the allosteric site of the enzyme. The binding of UDP-GlcNAc is associated with conformational changes in the active site of the enzyme. Kinetic data and mutagenesis of the highly conserved UDP-GlcNAc-interacting residues confirm their importance in the substrate binding and catalysis of the enzyme. This constitutes the first example to our knowledge, of an enzymatic allosteric activation by direct interaction between the substrate and the allosteric activator.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/3beo_consurf.spt"</scriptWhenChecked>
-
3BEO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BEO OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
A structural basis for the allosteric regulation of non-hydrolysing UDP-GlcNAc 2-epimerases., Velloso LM, Bhaskaran SS, Schuch R, Fischetti VA, Stebbins CE, EMBO Rep. 2008 Feb;9(2):199-205. Epub 2008 Jan 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18188181 18188181]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3beo ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Bacillus anthracis]]
[[Category: Bacillus anthracis]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: UDP-N-acetylglucosamine 2-epimerase]]
+
[[Category: Bhaskaran SS]]
-
[[Category: Bhaskaran, S S.]]
+
[[Category: Fischetti VA]]
-
[[Category: Fischetti, V A.]]
+
[[Category: Schuch R]]
-
[[Category: Schuch, R.]]
+
[[Category: Stebbins CE]]
-
[[Category: Stebbins, C E.]]
+
[[Category: Velloso LM]]
-
[[Category: Velloso, L M.]]
+
-
[[Category: Allosteric]]
+
-
[[Category: Epimerase]]
+
-
[[Category: Isomerase]]
+
-
[[Category: Regulation]]
+
-
[[Category: Udp-glcnac]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:41:28 2008''
+

Current revision

A Structural Basis for the allosteric regulation of non-hydrolyzing UDP-GlcNAc 2-epimerases

PDB ID 3beo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3beo"
Personal tools