3bfp

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[[Image:3bfp.jpg|left|200px]]
 
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==Crystal Structure of apo-PglD from Campylobacter jejuni==
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The line below this paragraph, containing "STRUCTURE_3bfp", creates the "Structure Box" on the page.
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<StructureSection load='3bfp' size='340' side='right'caption='[[3bfp]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BFP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
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{{STRUCTURE_3bfp| PDB=3bfp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bfp OCA], [https://pdbe.org/3bfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bfp RCSB], [https://www.ebi.ac.uk/pdbsum/3bfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bfp ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of apo-PglD from Campylobacter jejuni'''
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== Function ==
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[https://www.uniprot.org/uniprot/PGLD_CAMJE PGLD_CAMJE] Acetyltransferase that modifies the UDP-4-amino-sugar to form UDP-N,N'-diacetylbacillosamine in the N-linked protein glycosylation pathway.<ref>PMID:17087520</ref> <ref>PMID:19448740</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Campylobacter jejuni is highly unusual among bacteria in forming N-linked glycoproteins. The heptasaccharide produced by its pgl system is attached to protein Asn through its terminal 2,4-diacetamido-2,4,6-trideoxy-d-Glc (QuiNAc4NAc or N,N'-diacetylbacillosamine) moiety. The crucial, last part of this sugar's synthesis is the acetylation of UDP-2-acetamido-4-amino-2,4,6-trideoxy-d-Glc by the enzyme PglD, with acetyl-CoA as a cosubstrate. We have determined the crystal structures of PglD in CoA-bound and unbound forms, refined to 1.8 and 1.75 A resolution, respectively. PglD is a trimer of subunits each comprised of two domains, an N-terminal alpha/beta-domain and a C-terminal left-handed beta-helix. Few structural differences accompany CoA binding, except in the C-terminal region following the beta-helix (residues 189-195), which adopts an extended structure in the unbound form and folds to extend the beta-helix upon binding CoA. Computational molecular docking suggests a different mode of nucleotide-sugar binding with respect to the acetyl-CoA donor, with the molecules arranged in an "L-shape", compared with the "in-line" orientation in related enzymes. Modeling indicates that the oxyanion intermediate would be stabilized by the NH group of Gly143', with His125' the most likely residue to function as a general base, removing H+ from the amino group prior to nucleophilic attack at the carbonyl carbon of acetyl-CoA. Site-specific mutations of active site residues confirmed the importance of His125', Glu124', and Asn118. We conclude that Asn118 exerts its function by stabilizing the intricate hydrogen bonding network within the active site and that Glu124' may function to increase the pKa of the putative general base, His125'.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/3bfp_consurf.spt"</scriptWhenChecked>
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3BFP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BFP OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure and Active Site Residues of PglD, an N-Acetyltransferase from the Bacillosamine Synthetic Pathway Required for N-Glycan Synthesis in Campylobacter jejuni(,)., Rangarajan ES, Ruane KM, Sulea T, Watson DC, Proteau A, Leclerc S, Cygler M, Matte A, Young NM, Biochemistry. 2008 Feb 19;47(7):1827-1836. Epub 2008 Jan 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18198901 18198901]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bfp ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Campylobacter jejuni]]
[[Category: Campylobacter jejuni]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
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[[Category: Cygler M]]
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[[Category: Cygler, M.]]
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[[Category: Leclerc S]]
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[[Category: Leclerc, S.]]
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[[Category: Matte A]]
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[[Category: Matte, A.]]
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[[Category: Proteau A]]
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[[Category: Proteau, A.]]
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[[Category: Rangarajan ES]]
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[[Category: Rangarajan, E S.]]
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[[Category: Watson DC]]
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[[Category: Watson, D C.]]
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[[Category: Young NM]]
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[[Category: Young, N M.]]
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[[Category: Bacillosamine]]
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[[Category: Bsgi]]
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[[Category: Coa binding protein]]
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[[Category: Left-handed beta helix]]
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[[Category: Mkbsgi]]
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[[Category: Montreal-kingston bacterial structural genomics initiative]]
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[[Category: N-acetyltransferase]]
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[[Category: N-glycan biosynthesis]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:43:18 2008''
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Current revision

Crystal Structure of apo-PglD from Campylobacter jejuni

PDB ID 3bfp

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