1arf

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(New page: 200px<br /><applet load="1arf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1arf" /> '''STRUCTURES OF DNA-BINDING MUTANT ZINC FINGER...)
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[[Image:1arf.gif|left|200px]]<br /><applet load="1arf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1arf" />
 
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'''STRUCTURES OF DNA-BINDING MUTANT ZINC FINGER DOMAINS: IMPLICATIONS FOR DNA BINDING'''<br />
 
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==Overview==
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==STRUCTURES OF DNA-BINDING MUTANT ZINC FINGER DOMAINS: IMPLICATIONS FOR DNA BINDING==
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Studies of Cys2-His2 zinc finger domains have revealed that the structures, of individual finger domains in solution determined by NMR spectroscopy, are strikingly similar to the structure of fingers bound to DNA determined, by X-ray diffraction. Therefore, detailed structural analyses of single, finger domains that contain amino acid substitutions known to affect DNA, binding in the whole protein can yield information concerning the, structural ramifications of such mutations. We have used this approach to, study two mutants in the N-terminal finger domain of ADR1, a yeast, transcription factor that contains two Cys2-His2 zinc finger sequences, spanning residues 102-159. Two point mutants at position 118 in the, N-terminal zinc finger (ADR1b: 102-130) that adversely affect the, DNA-binding activity of ADR1 have previously been identified: H118A and, H118Y. The structures of wild-type ADR1b and the two mutant zinc finger, domains were determined using two-dimensional nuclear magnetic resonance, spectroscopy and distance geometry and were refined using a complete, relaxation matrix method approach (REPENT) to improve agreement between, the models and the nuclear Overhauser effect spectroscopy data from which, they were generated. The molecular architecture of the refined wild-type, ADR1b domain is presented in detail. Comparisons of wild-type ADR1b and, the two mutants revealed that neither mutation causes a significant, structural perturbation. The structures indicate that the DNA binding, properties of the His 118 mutants are dependent on the identity of the, side chain at position 118, which has been postulated to make a direct DNA, contact in the wild-type ADR1 protein. The results suggest that the, identity of the side chain at the middle DNA contact position in Cys2-His2, zinc fingers may be changed with impunity regarding the domain structure, and can affect the affinity of the protein-DNA interaction.
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<StructureSection load='1arf' size='340' side='right'caption='[[1arf]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1arf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ARF FirstGlance]. <br>
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1ARF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ARF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1arf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1arf OCA], [https://pdbe.org/1arf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1arf RCSB], [https://www.ebi.ac.uk/pdbsum/1arf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1arf ProSAT]</span></td></tr>
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Structures of DNA-binding mutant zinc finger domains: implications for DNA binding., Hoffman RC, Horvath SJ, Klevit RE, Protein Sci. 1993 Jun;2(6):951-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8318900 8318900]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ADR1_YEAST ADR1_YEAST] Required for transcriptional activation of glucose-repressible alcohol dehydrogenase (ADH2).
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Herriott JR]]
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[[Category: Herriott, J.R.]]
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[[Category: Hoffman RC]]
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[[Category: Hoffman, R.C.]]
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[[Category: Horvath SJ]]
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[[Category: Horvath, S.J.]]
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[[Category: Klevit RE]]
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[[Category: Klevit, R.E.]]
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[[Category: Xu RX]]
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[[Category: Xu, R.X.]]
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[[Category: ZN]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:04:20 2007''
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Current revision

STRUCTURES OF DNA-BINDING MUTANT ZINC FINGER DOMAINS: IMPLICATIONS FOR DNA BINDING

PDB ID 1arf

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