1aua

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(New page: 200px<br /><applet load="1aua" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aua, resolution 2.5&Aring;" /> '''PHOSPHATIDYLINOSITOL ...)
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[[Image:1aua.gif|left|200px]]<br /><applet load="1aua" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aua, resolution 2.5&Aring;" />
 
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'''PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE'''<br />
 
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==Overview==
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==PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE==
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The yeast phosphatidylinositol-transfer protein (Sec14) catalyses exchange, of phosphatidylinositol and phosphatidylcholine between membrane bilayers, in vitro. In vivo, Sec14 activity is essential for vesicle budding from, the Golgi complex. Here we report a three-dimensional structure for Sec14, at 2.5 A resolution. Sec14 consists of twelve alpha-helices, six, beta-strands, eight 3(10)-helices and has two distinct domains. The, carboxy-terminal domain forms a hydrophobic pocket which, in the crystal, structure, is occupied by two molecules of n-octyl-beta-D-glucopyranoside, and represents the phospholipid-binding domain. This pocket is reinforced, by a string motif whose disruption in a sec14 temperature-sensitive mutant, results in destabilization of the phospholipid-binding domain. Finally, we, have identified an unusual surface helix that may play a critical role in, driving Sec14-mediated phospholipid exchange. From this structure, we, derive the first molecular clues into how a phosphatidylinositol-transfer, protein functions.
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<StructureSection load='1aua' size='340' side='right'caption='[[1aua]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1aua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AUA FirstGlance]. <br>
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1AUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with BOG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AUA OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aua OCA], [https://pdbe.org/1aua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aua RCSB], [https://www.ebi.ac.uk/pdbsum/1aua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aua ProSAT]</span></td></tr>
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Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein., Sha B, Phillips SE, Bankaitis VA, Luo M, Nature. 1998 Jan 29;391(6666):506-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9461221 9461221]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SEC14_YEAST SEC14_YEAST] Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1aua_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aua ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Bankaitis VA]]
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[[Category: Bankaitis, V.A.]]
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[[Category: Luo M]]
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[[Category: Luo, M.]]
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[[Category: Phillips SE]]
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[[Category: Phillips, S.E.]]
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[[Category: Sha B]]
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[[Category: Sha, B.]]
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[[Category: BOG]]
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[[Category: golgi-derived secretory vesicle biogenesis]]
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[[Category: peripheral golgi membrane protein]]
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[[Category: phospholipid exchange]]
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[[Category: phospholipid-binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:08:13 2007''
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Current revision

PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE

PDB ID 1aua

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