1aw2

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(New page: 200px<br /><applet load="1aw2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aw2, resolution 2.65&Aring;" /> '''TRIOSEPHOSPHATE ISOM...)
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[[Image:1aw2.gif|left|200px]]<br /><applet load="1aw2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aw2, resolution 2.65&Aring;" />
 
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'''TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS'''<br />
 
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==Overview==
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==TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS==
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The purification and characterization of triose-phosphate isomerase from, the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal, structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate, complex (at a 2.7-A resolution) are also presented. The optimal growth, temperature of Vibrio marinus is 15 degrees C. Stability studies show that, vTIM is an unstable protein with a half-life of only 10 min at 25 degrees, C. The vTIM sequence is most closely related to the sequence of, Escherichia coli TIM (eTIM) (66% identity), and several unique structural, features described for eTIM are also seen in vTIM, but eTIM is, considerably more stable. The Td values of vTIM and eTIM, determined by, calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid, sequence comparison reveals that vTIM has an alanine in loop 8 (at, position 238), whereas all other TIM sequences known to date have a, serine. The vTIM mutant A238S was produced and characterized. Compared, with wild type, the catalytic efficiency of the A238S mutant is somewhat, reduced, and its stability is considerably increased.
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<StructureSection load='1aw2' size='340' side='right'caption='[[1aw2]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1aw2]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Moritella_marina Moritella marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AW2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AW2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aw2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aw2 OCA], [https://pdbe.org/1aw2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aw2 RCSB], [https://www.ebi.ac.uk/pdbsum/1aw2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aw2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TPIS_MORMI TPIS_MORMI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aw/1aw2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aw2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The purification and characterization of triose-phosphate isomerase from the psychrophilic bacterium Vibrio marinus (vTIM) is described. Crystal structures of the vTIM-sulfate complex and the vTIM-2-phosphoglycolate complex (at a 2.7-A resolution) are also presented. The optimal growth temperature of Vibrio marinus is 15 degrees C. Stability studies show that vTIM is an unstable protein with a half-life of only 10 min at 25 degrees C. The vTIM sequence is most closely related to the sequence of Escherichia coli TIM (eTIM) (66% identity), and several unique structural features described for eTIM are also seen in vTIM, but eTIM is considerably more stable. The Td values of vTIM and eTIM, determined by calorimetric studies, are 41 and 54 degrees C, respectively. Amino acid sequence comparison reveals that vTIM has an alanine in loop 8 (at position 238), whereas all other TIM sequences known to date have a serine. The vTIM mutant A238S was produced and characterized. Compared with wild type, the catalytic efficiency of the A238S mutant is somewhat reduced, and its stability is considerably increased.
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==About this Structure==
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Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties.,Alvarez M, Zeelen JP, Mainfroid V, Rentier-Delrue F, Martial JA, Wyns L, Wierenga RK, Maes D J Biol Chem. 1998 Jan 23;273(4):2199-206. PMID:9442062<ref>PMID:9442062</ref>
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1AW2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moritella_marina Moritella marina] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AW2 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Triose-phosphate isomerase (TIM) of the psychrophilic bacterium Vibrio marinus. Kinetic and structural properties., Alvarez M, Zeelen JP, Mainfroid V, Rentier-Delrue F, Martial JA, Wyns L, Wierenga RK, Maes D, J Biol Chem. 1998 Jan 23;273(4):2199-206. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9442062 9442062]
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</div>
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[[Category: Moritella marina]]
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<div class="pdbe-citations 1aw2" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Triose-phosphate isomerase]]
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[[Category: Maes, D.]]
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[[Category: Wierenga, R.K.]]
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[[Category: Zeelen, J.P.]]
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[[Category: SO4]]
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[[Category: isomerase]]
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[[Category: psychrophilic]]
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[[Category: vibrio marinus]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:10:08 2007''
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==See Also==
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*[[Triose phosphate isomerase 3D structures|Triose phosphate isomerase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Moritella marina]]
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[[Category: Maes D]]
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[[Category: Wierenga RK]]
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[[Category: Zeelen JP]]

Current revision

TRIOSEPHOSPHATE ISOMERASE OF VIBRIO MARINUS

PDB ID 1aw2

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