|
|
| (7 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:409d.gif|left|200px]] | |
| | | | |
| - | <!--
| + | ==CRYSTAL STRUCTURE OF AN RNA R(CCCIUGGG) WITH THREE INDEPENDENT DUPLEXES INCORPORATING TANDEM I.U WOBBLES== |
| - | The line below this paragraph, containing "STRUCTURE_409d", creates the "Structure Box" on the page.
| + | <StructureSection load='409d' size='340' side='right'caption='[[409d]], [[Resolution|resolution]] 2.50Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[409d]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=409D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=409D FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=409d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=409d OCA], [https://pdbe.org/409d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=409d RCSB], [https://www.ebi.ac.uk/pdbsum/409d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=409d ProSAT]</span></td></tr> |
| - | {{STRUCTURE_409d| PDB=409d | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''CRYSTAL STRUCTURE OF AN RNA R(CCCIUGGG) WITH THREE INDEPENDENT DUPLEXES INCORPORATING TANDEM I.U WOBBLES'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Hart D]] |
| - | ==Overview==
| + | [[Category: Mitra SN]] |
| - | The crystal structure of the RNA octamer duplex r(CCCIUGGG)2has been elucidated at 2.5 A resolution. The crystals belong to the space group P21and have unit cell constants a = 33.44 A, b = 43.41 A, c = 49.39 A and beta = 104.7 degrees with three independent duplexes (duplexes 1-3) in the asymmetric unit. The structure was solved by the molecular replacement method and refined to an Rwork/Rfree of 0.185/0.243 using 3765 reflections between 8.0 and 2.5 A. This is the first report of an RNA crystal structure incorporating I.U wobbles and three molecules in the asymmetric unit. Duplex 1 displays a kink of 24 degrees between the mismatch sites, while duplexes 2 and 3 have two kinks each of 19 degrees and 27 degrees, and 24 degrees and 29 degrees, respectively, on either side of the tandem mismatches. At the I.U/U.I mismatch steps, duplex 1 has a twist angle of 33.9 degrees, close to the average for all base pair steps, but duplexes 2 and 3 are underwound, with twist angles of 24.4 degrees and 26.5 degrees, respectively. The tandem I.U wobbles show intrastrand purine-pyrimidine stacking but exhibit interstrand purine-purine stacking with the flanking C.G pairs. The three independent duplexes are stacked non-coaxially in a head-to-tail fashion to form infinite pseudo-continuous helical columns which form intercolumn hydrogen bonding interactions through the 2'-hydroxyl groups where the minor grooves come together.
| + | [[Category: Pan B]] |
| - | | + | [[Category: Sun L]] |
| - | ==About this Structure== | + | [[Category: Sundaralingam M]] |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=409D OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Crystal structure of an RNA octamer duplex r(CCCIUGGG)2 incorporating tandem I.U wobbles., Pan B, Mitra SN, Sun L, Hart D, Sundaralingam M, Nucleic Acids Res. 1998 Dec 15;26(24):5699-706. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9838002 9838002]
| + | |
| - | [[Category: Hart, D.]]
| + | |
| - | [[Category: Mitra, S N.]] | + | |
| - | [[Category: Pan, B.]] | + | |
| - | [[Category: Sun, L.]] | + | |
| - | [[Category: Sundaralingam, M.]] | + | |
| - | [[Category: Double helix]] | + | |
| - | [[Category: I u wobble]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:16:26 2008''
| + | |
