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| - | [[Image:431d.gif|left|200px]] | |
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| - | <!--
| + | ==5'-D(*GP*GP*CP*CP*AP*AP*TP*TP*GP*G)-3'== |
| - | The line below this paragraph, containing "STRUCTURE_431d", creates the "Structure Box" on the page.
| + | <StructureSection load='431d' size='340' side='right'caption='[[431d]], [[Resolution|resolution]] 1.15Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[431d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=431D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=431D FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15Å</td></tr> |
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| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | {{STRUCTURE_431d| PDB=431d | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=431d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=431d OCA], [https://pdbe.org/431d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=431d RCSB], [https://www.ebi.ac.uk/pdbsum/431d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=431d ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''5'-D(*GP*GP*CP*CP*AP*AP*TP*TP*GP*G)-3''''
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview== | + | [[Category: Turkenburg JP]] |
| - | Despite the importance of hydration around DNA in the understanding of its conformation and interactions with other molecules in many biological processes, only limited atomic resolution information is available. Crystal-engineering techniques, which were originally developed to mimic DNA base triplets in a crystal lattice, also eliminate the rotational disorder of oligonucleotides around their helical axis and thereby enhance the resolution of the structure analysis. We have determined the low-temperature crystal structure of the synthetic DNA decamer d(GGCCAATTGG) at atomic resolution (1. 15 A) using 17700 reflections and have characterized the highly organized hydration patterns in both grooves. The narrow d(AATT) minor groove is occupied by an 'extended hydration spine' alternately bridging base pairs and phosphate O1P atoms of opposite strands, while a distinctive pattern of parallel water ribbons is observed in the major groove. This analysis provides structural insight into the correlation found between narrow minor-groove width and occurrence of the B(I) conformation and can be used to design new minor-groove binders. By their location between adjacent helices, two fully hydrated magnesium ions further stabilize the crystal packing. The structure also provides details of the hydration and conformation of G.GC triple helices.
| + | [[Category: Van Meervelt L]] |
| - | | + | [[Category: Vlieghe D]] |
| - | ==About this Structure== | + | |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=431D OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | B-DNA at atomic resolution reveals extended hydration patterns., Vlieghe D, Turkenburg JP, Van Meervelt L, Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1495-502. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10489444 10489444]
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| - | [[Category: Meervelt, L Van.]]
| + | |
| - | [[Category: Turkenburg, J P.]] | + | |
| - | [[Category: Vlieghe, D.]] | + | |
| - | [[Category: Atomic resolution]] | + | |
| - | [[Category: Deoxyribonucleic acid]] | + | |
| - | [[Category: Double backbone conformation]] | + | |
| - | [[Category: Extended hydration spine]] | + | |
| - | [[Category: Triplet formation]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:17:25 2008''
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