463d

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[[Image:463d.gif|left|200px]]
 
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==CALCIUM FORM OF D(CGCGAATTCGCG)2==
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The line below this paragraph, containing "STRUCTURE_463d", creates the "Structure Box" on the page.
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<StructureSection load='463d' size='340' side='right'caption='[[463d]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[463d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=463D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=463D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_463d| PDB=463d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=463d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=463d OCA], [https://pdbe.org/463d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=463d RCSB], [https://www.ebi.ac.uk/pdbsum/463d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=463d ProSAT]</span></td></tr>
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</table>
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'''CALCIUM FORM OF D(CGCGAATTCGCG)2'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Liu J]]
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The dodecamer d(CGCGAATTCGCG) was the first oligonucleotide to be crystallized as a B-DNA duplex. Its structure was analyzed in detail in the early 1980s. Here we show that, in the presence of Ca(2+), it crystallizes in a different way (R3 space group). The dodecamers form parallel columns of straight duplexes with ten base pairs in the B form. The terminal cytosines in each molecule are disordered, whereas the terminal guanines are placed in the minor groove of neighbor duplexes. The central GAATTC region is practically identical to that found in the classic structure of the same dodecamer crystallized in the P2(1)2(1)2(1) space group in the presence of Mg(2+) and spermine. Its structure is thus independent of the crystallization conditions which have been used.
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[[Category: Subirana JA]]
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==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=463D OCA].
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==Reference==
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Structure of d(CGCGAATTCGCG) in the presence of Ca(2+) ions., Liu J, Subirana JA, J Biol Chem. 1999 Aug 27;274(35):24749-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10455145 10455145]
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[[Category: Liu, J.]]
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[[Category: Subirana, J A.]]
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[[Category: Ca2+ binding]]
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[[Category: Dickerson-drew dodecamer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:19:08 2008''
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Current revision

CALCIUM FORM OF D(CGCGAATTCGCG)2

PDB ID 463d

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