4hb1

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[[Image:4hb1.jpg|left|200px]]
 
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==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==
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The line below this paragraph, containing "STRUCTURE_4hb1", creates the "Structure Box" on the page.
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<StructureSection load='4hb1' size='340' side='right'caption='[[4hb1]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
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{{STRUCTURE_4hb1| PDB=4hb1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [https://pdbe.org/4hb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>
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</table>
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'''A DESIGNED FOUR HELIX BUNDLE PROTEIN.'''
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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==Overview==
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[[Category: Synthetic construct]]
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A 108 amino acid protein was designed and constructed from a reduced alphabet of seven amino acids. The 2.9 A resolution X-ray crystal structure confirms that the protein is a four helix bundle, as it was designed to be. Hydrogen/deuterium exchange experiments reveal buried amide protons with protection factors in excess of 1 x 10(6) in the range characteristic of well protected protons in functional folded proteins (10(3)-10(8)) rather than protons in rapid exchange (0-10(2)). The protein is monomeric at 1 mM, the concentration at which the exchange experiments were undertaken, indicating that the exchange factors are due to a unique stable tertiary structure fold, and not due to any higher order quaternary structure. Thermodynamic analysis provides an estimate of the free energy of folding of -9.3 kcal mole-1 at 25 degrees C, consistent with the free energy of folding derived from the protection factors of the most protected protons, indicating that global unfolding is required for exchange of the most protected protons.
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[[Category: Laporte SL]]
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[[Category: Miercke LJW]]
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==About this Structure==
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[[Category: Schafmeister CE]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].
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[[Category: Stroud RM]]
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==Reference==
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A designed four helix bundle protein with native-like structure., Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM, Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9406555 9406555]
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[[Category: Laporte, S L.]]
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[[Category: Miercke, L J.W.]]
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[[Category: Schafmeister, C E.]]
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[[Category: Stroud, R M.]]
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[[Category: Designed helical bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:25:05 2008''
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Current revision

A DESIGNED FOUR HELIX BUNDLE PROTEIN.

PDB ID 4hb1

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