4rnp

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[[Image:4rnp.jpg|left|200px]]
 
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==BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY==
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The line below this paragraph, containing "STRUCTURE_4rnp", creates the "Structure Box" on the page.
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<StructureSection load='4rnp' size='340' side='right'caption='[[4rnp]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4rnp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rnp 3rnp] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2rnp 2rnp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rnp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnp OCA], [https://pdbe.org/4rnp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rnp RCSB], [https://www.ebi.ac.uk/pdbsum/4rnp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnp ProSAT]</span></td></tr>
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{{STRUCTURE_4rnp| PDB=4rnp | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rn/4rnp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4rnp ConSurf].
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<div style="clear:both"></div>
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'''BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY'''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structure of T7 RNA polymerase reveals a molecule organized around a cleft that can accommodate a double-stranded DNA template. A portion (approximately 45%) of the molecule displays extensive structural homology to the polymerase domain of Klenow fragment and more limited homology to the human immunodeficiency virus HIV-1 reverse transcriptase. A comparison of the structures and sequences of these polymerases identifies structural elements that may be responsible for discriminating between ribonucleotide and deoxyribonucleotide substrates, and RNA and DNA templates. The relative locations of the catalytic site and a specific promoter recognition residue allow the orientation of the polymerase on the template to be defined.
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[[Category: Escherichia phage T7]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Liu ZJ]]
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4RNP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. This structure supersedes the now removed PDB entries and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2rnp 2rnp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNP OCA].
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[[Category: Rose JP]]
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[[Category: Sousa R]]
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==Reference==
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[[Category: Wang BC]]
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Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution., Sousa R, Chung YJ, Rose JP, Wang BC, Nature. 1993 Aug 12;364(6438):593-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7688864 7688864]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Enterobacteria phage t7]]
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[[Category: Single protein]]
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[[Category: Liu, Z J.]]
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[[Category: Rose, J P.]]
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[[Category: Sousa, R.]]
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[[Category: Wang, B C.]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Rna polymerase]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:29:00 2008''
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Current revision

BACTERIOPHAGE T7 RNA POLYMERASE, HIGH SALT CRYSTAL FORM, LOW TEMPERATURE DATA, ALPHA-CARBONS ONLY

PDB ID 4rnp

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