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6ins

From Proteopedia

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[[Image:6ins.gif|left|200px]]
 
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==X-RAY ANALYSIS OF THE SINGLE CHAIN B29-A1 PEPTIDE-LINKED INSULIN MOLECULE. A COMPLETELY INACTIVE ANALOGUE==
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The line below this paragraph, containing "STRUCTURE_6ins", creates the "Structure Box" on the page.
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<StructureSection load='6ins' size='340' side='right' caption='[[6ins]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[6ins]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pig Pig]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6INS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6INS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ins FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ins OCA], [http://pdbe.org/6ins PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ins RCSB], [http://www.ebi.ac.uk/pdbsum/6ins PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ins ProSAT]</span></td></tr>
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{{STRUCTURE_6ins| PDB=6ins | SCENE= }}
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/INS_PIG INS_PIG]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/6ins_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=6ins ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A crystal structure of a totally inactive insulin molecule has been determined. For this insulin molecule, the first without detectable activity to be characterized, the A and B-chains are linked by a peptide bond between A1 Gly and B29 Lys. The molecule has retained all its normal self-association properties and it can also accommodate the two different conformations designated T and R, as seen in 4Zn native pig insulin crystals. The hexamers of the crosslinked insulin molecule were crystallized using the 4Zn insulin recipe of Schlichtkrull. The structure has been crystallographically refined with data extending to 2 A using restrained least-square methods. Comparison of the B29-A1 peptide crosslink insulin and the 4Zn native insulin reveals close structural similarities with the native dimer. The analysis of the structure confirms the earlier hypothesis that insulin structures in crystals are not in an active conformation and that a separation of N-terminal A-chain and C-terminal B-chain is required for interaction with the insulin receptor.
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'''X-RAY ANALYSIS OF THE SINGLE CHAIN /B29-A1$ PEPTIDE-LINKED INSULIN MOLECULE. A COMPLETELY INACTIVE ANALOGUE'''
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X-ray analysis of the single chain B29-A1 peptide-linked insulin molecule. A completely inactive analogue.,Derewenda U, Derewenda Z, Dodson EJ, Dodson GG, Bing X, Markussen J J Mol Biol. 1991 Jul 20;220(2):425-33. PMID:1856866<ref>PMID:1856866</ref>
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==Overview==
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A crystal structure of a totally inactive insulin molecule has been determined. For this insulin molecule, the first without detectable activity to be characterized, the A and B-chains are linked by a peptide bond between A1 Gly and B29 Lys. The molecule has retained all its normal self-association properties and it can also accommodate the two different conformations designated T and R, as seen in 4Zn native pig insulin crystals. The hexamers of the crosslinked insulin molecule were crystallized using the 4Zn insulin recipe of Schlichtkrull. The structure has been crystallographically refined with data extending to 2 A using restrained least-square methods. Comparison of the B29-A1 peptide crosslink insulin and the 4Zn native insulin reveals close structural similarities with the native dimer. The analysis of the structure confirms the earlier hypothesis that insulin structures in crystals are not in an active conformation and that a separation of N-terminal A-chain and C-terminal B-chain is required for interaction with the insulin receptor.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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6INS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6INS OCA].
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</div>
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<div class="pdbe-citations 6ins" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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X-ray analysis of the single chain B29-A1 peptide-linked insulin molecule. A completely inactive analogue., Derewenda U, Derewenda Z, Dodson EJ, Dodson GG, Bing X, Markussen J, J Mol Biol. 1991 Jul 20;220(2):425-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1856866 1856866]
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*[[Molecular Playground/Insulin|Molecular Playground/Insulin]]
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[[Category: Single protein]]
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== References ==
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[[Category: Sus scrofa]]
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<references/>
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[[Category: Bing, X.]]
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__TOC__
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[[Category: Derewenda, U.]]
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</StructureSection>
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[[Category: Derewenda, Z.]]
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[[Category: Pig]]
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[[Category: Dodson, E J.]]
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[[Category: Bing, X]]
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[[Category: Dodson, G G.]]
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[[Category: Derewenda, U]]
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[[Category: Markussen, J.]]
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[[Category: Derewenda, Z]]
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[[Category: Dodson, E J]]
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[[Category: Dodson, G G]]
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[[Category: Markussen, J]]
[[Category: Hormone]]
[[Category: Hormone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:41:28 2008''
 

Current revision

X-RAY ANALYSIS OF THE SINGLE CHAIN B29-A1 PEPTIDE-LINKED INSULIN MOLECULE. A COMPLETELY INACTIVE ANALOGUE

6ins, resolution 2.00Å

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