7ins

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[[Image:7ins.gif|left|200px]]
 
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==STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z==
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The line below this paragraph, containing "STRUCTURE_7ins", creates the "Structure Box" on the page.
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<StructureSection load='7ins' size='340' side='right'caption='[[7ins]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[7ins]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7INS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7INS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRS:M-CRESOL'>CRS</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_7ins| PDB=7ins | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ins FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ins OCA], [https://pdbe.org/7ins PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ins RCSB], [https://www.ebi.ac.uk/pdbsum/7ins PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ins ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INS_PIG INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/7ins_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7ins ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods. The final crystallographic R factor for all reflections between 2 and 10 A is 19.4%. The insulin molecules are arranged as hexamers with two tetrahedrally coordinated Zn atoms in the central channel and one m-cresol bound to each monomer near His B5. One protamine binding site has been unequivocally identified near a dimer-dimer interface, although most of the polypeptide is crystallographically disordered. The conformation of the insulin moiety and the structural differences between the three unique monomers have been analysed. The zinc and m-cresol environments are described and the nature of the protamine binding site is outlined.
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'''STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z'''
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Structure of porcine insulin cocrystallized with clupeine Z.,Balschmidt P, Hansen FB, Dodson EJ, Dodson GG, Korber F Acta Crystallogr B. 1991 Dec 1;47 ( Pt 6):975-86. PMID:1772633<ref>PMID:1772633</ref>
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==Overview==
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The crystal structure of NPH-insulin, pig insulin cocrystallized with zinc, m-cresol and protamine, has been solved by molecular replacement and refined using restrained least-squares refinement methods. The final crystallographic R factor for all reflections between 2 and 10 A is 19.4%. The insulin molecules are arranged as hexamers with two tetrahedrally coordinated Zn atoms in the central channel and one m-cresol bound to each monomer near His B5. One protamine binding site has been unequivocally identified near a dimer-dimer interface, although most of the polypeptide is crystallographically disordered. The conformation of the insulin moiety and the structural differences between the three unique monomers have been analysed. The zinc and m-cresol environments are described and the nature of the protamine binding site is outlined.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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7INS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7INS OCA].
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</div>
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<div class="pdbe-citations 7ins" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure of porcine insulin cocrystallized with clupeine Z., Balschmidt P, Hansen FB, Dodson EJ, Dodson GG, Korber F, Acta Crystallogr B. 1991 Dec 1;47 ( Pt 6):975-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1772633 1772633]
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*[[Insulin 3D Structures|Insulin 3D Structures]]
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[[Category: Protein complex]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Balschmidt, P.]]
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[[Category: Balschmidt P]]
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[[Category: Dodson, E.]]
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[[Category: Dodson E]]
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[[Category: Dodson, G.]]
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[[Category: Dodson G]]
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[[Category: Hansen, F B.]]
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[[Category: Hansen FB]]
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[[Category: Korber, F.]]
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[[Category: Korber F]]
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[[Category: Hormone]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:46:20 2008''
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STRUCTURE OF PORCINE INSULIN COCRYSTALLIZED WITH CLUPEINE Z

PDB ID 7ins

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