2r5i

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2r5i", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
Current revision (09:19, 21 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2r5i.jpg|left|200px]]
 
-
<!--
+
==Pentamer Structure of Major Capsid Protein L1 of Human Papilloma Virus type 18==
-
The line below this paragraph, containing "STRUCTURE_2r5i", creates the "Structure Box" on the page.
+
<StructureSection load='2r5i' size='340' side='right'caption='[[2r5i]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2r5i]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_papillomavirus_type_18 Human papillomavirus type 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5I FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5i OCA], [https://pdbe.org/2r5i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5i RCSB], [https://www.ebi.ac.uk/pdbsum/2r5i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5i ProSAT]</span></td></tr>
-
{{STRUCTURE_2r5i| PDB=2r5i | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/VL1_HPV18 VL1_HPV18] Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.[HAMAP-Rule:MF_04002]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5i_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5i ConSurf].
 +
<div style="clear:both"></div>
-
'''Pentamer Structure of Major Capsid Protein L1 of Human Papilloma Virus type 18'''
+
==See Also==
-
 
+
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
Human papillomaviruses (HPVs) are known etiologic agents of cervical cancer. Vaccines that contain virus-like particles (VLPs) made of L1 capsid protein from several high risk HPV types have proven to be effective against HPV infections. Raising high levels of neutralizing antibodies against each HPV type is believed to be the primary mechanism of protection, gained by vaccination. Antibodies elicited by a particular HPV type are highly specific to that particular HPV type and show little or no cross-reactivity between HPV types. With an intention to understand the interplay between the L1 structure of different HPV types and the type specificity of neutralizing antibodies, we have prepared the L1 pentamers of four different HPV types, HPV11, HPV16, HPV18, and HPV35. The pentamers only bind the type-specific neutralizing monoclonal antibodies (NmAbs) that are raised against the VLP of the corresponding HPV type, implying that the surface loop structures of the pentamers from each type are distinctive and functionally active as VLPs in terms of antibody binding. We have determined the crystal structures of all four L1 pentamers, and their comparisons revealed characteristic conformational differences of the surface loops that contain the known epitopes for the NmAbs. On the basis of these distinct surface loop structures, we have provided a molecular explanation for the type specificity of NmAbs against HPV infection.
+
-
 
+
-
==About this Structure==
+
-
2R5I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_18 Human papillomavirus type 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5I OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structures of four types of human papillomavirus L1 capsid proteins: understanding the specificity of neutralizing monoclonal antibodies., Bishop B, Dasgupta J, Klein M, Garcea RL, Christensen ND, Zhao R, Chen XS, J Biol Chem. 2007 Oct 26;282(43):31803-11. Epub 2007 Sep 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17804402 17804402]
+
[[Category: Human papillomavirus type 18]]
[[Category: Human papillomavirus type 18]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bishop, B.]]
+
[[Category: Bishop B]]
-
[[Category: Chen, X S.]]
+
[[Category: Chen XS]]
-
[[Category: Dasgupta, J.]]
+
[[Category: Dasgupta J]]
-
[[Category: Capsid]]
+
-
[[Category: Capsid protein]]
+
-
[[Category: Hpv18]]
+
-
[[Category: Pentamer]]
+
-
[[Category: Structure]]
+
-
[[Category: Type specific epitope]]
+
-
[[Category: Viral protein]]
+
-
[[Category: Virion]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:42:16 2008''
+

Current revision

Pentamer Structure of Major Capsid Protein L1 of Human Papilloma Virus type 18

PDB ID 2r5i

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2r5i"
Personal tools