|
|
| (9 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:2zkq.jpg|left|200px]] | + | #REDIRECT [[4v5z]] This PDB entry is obsolete and replaced by 4v5z |
| - | | + | |
| - | <!--
| + | |
| - | The line below this paragraph, containing "STRUCTURE_2zkq", creates the "Structure Box" on the page.
| + | |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | |
| - | or leave the SCENE parameter empty for the default display.
| + | |
| - | -->
| + | |
| - | {{STRUCTURE_2zkq| PDB=2zkq | SCENE= }}
| + | |
| - | | + | |
| - | '''Structure of a mammalian Ribosomal 40S subunit within an 80S complex obtained by docking homology models of the RNA and proteins into an 8.7 A cryo-EM map'''
| + | |
| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | In this paper, we present a structure of the mammalian ribosome determined at approximately 8.7 A resolution by electron cryomicroscopy and single-particle methods. A model of the ribosome was created by docking homology models of subunit rRNAs and conserved proteins into the density map. We then modeled expansion segments in the subunit rRNAs and found unclaimed density for approximately 20 proteins. In general, many conserved proteins and novel proteins interact with expansion segments to form an integrated framework that may stabilize the mature ribosome. Our structure provides a snapshot of the mammalian ribosome at the beginning of translation and lends support to current models in which large movements of the small subunit and L1 stalk occur during tRNA translocation. Finally, details are presented for intersubunit bridges that are specific to the eukaryotic ribosome. We suggest that these bridges may help reset the conformation of the ribosome to prepare for the next cycle of chain elongation.
| + | |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 2ZKQ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZKQ OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | Structure of the Mammalian 80S Ribosome at 8.7 A Resolution., Chandramouli P, Topf M, Menetret JF, Eswar N, Cannone JJ, Gutell RR, Sali A, Akey CW, Structure. 2008 Apr;16(4):535-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18400176 18400176]
| + | |
| - | [[Category: Canis lupus familiaris]]
| + | |
| - | [[Category: Protein complex]]
| + | |
| - | [[Category: Akey, C W.]]
| + | |
| - | [[Category: Chandramouli, P.]]
| + | |
| - | [[Category: 40s ribosomal subunit]]
| + | |
| - | [[Category: Protein-rna complex]]
| + | |
| - | [[Category: Ribosomal protein/rna complex]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:51:51 2008''
| + | |
