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1b54

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(New page: 200px<br /><applet load="1b54" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b54, resolution 2.1&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1b54.gif|left|200px]]<br /><applet load="1b54" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1b54, resolution 2.1&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A YEAST HYPOTHETICAL PROTEIN-A STRUCTURE FROM BNL'S HUMAN PROTEOME PROJECT'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A YEAST HYPOTHETICAL PROTEIN-A STRUCTURE FROM BNL'S HUMAN PROTEOME PROJECT==
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Yeast hypothetical protein YBL036C (SWISS-PROT P38197), initially thought, to be a member of an 11-protein family, was selected for crystal structure, determination since no structural or functional information was available., The structure has been determined independently by MIR and MAD methods to, 2.0 A resolution. The MAD structure was determined largely through, automated model building. The protein folds as a TIM barrel beginning with, a long N-terminal helix, in contrast to the classic triose phosphate, isomerase (TIM) structure, which begins with a beta-strand. A cofactor, pyridoxal 5'-phosphate, is covalently bound near the C-terminal end of the, barrel, the usual active site in TIM-barrel folds. A single-domain, monomeric molecule, this yeast protein resembles the N-terminal domain of, alanine racemase or ornithine decarboxylase, both of which are two-domain, dimeric proteins. The yeast protein has been shown to have amino-acid, racemase activity. Although selected as a member of a protein family, having no obvious relationship to proteins of known structure, the protein, fold turned out to be a well known and widely distributed fold. This, points to the need for a more comprehensive base of structural information, and better structure-modeling tools before the goal of structure, prediction from amino-acid sequences can be realised. In this case, similarity to a known structure allowed inferences to be made about the, structure and function of a widely distributed protein family.
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<StructureSection load='1b54' size='340' side='right'caption='[[1b54]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1b54]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B54 FirstGlance]. <br>
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1B54 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with PLP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B54 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b54 OCA], [https://pdbe.org/1b54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b54 RCSB], [https://www.ebi.ac.uk/pdbsum/1b54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b54 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1b54 TOPSAN]</span></td></tr>
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Structure of a yeast hypothetical protein selected by a structural genomics approach., Eswaramoorthy S, Gerchman S, Graziano V, Kycia H, Studier FW, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):127-35. Epub 2002, Dec 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12499548 12499548]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLPHP_YEAST PLPHP_YEAST] Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.[HAMAP-Rule:MF_03225]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/1b54_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b54 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Burley SK]]
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[[Category: Burley, S.K.]]
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[[Category: Eswaramoorthy S]]
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[[Category: Eswaramoorthy, S.]]
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[[Category: Swaminathan S]]
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[[Category: NYSGXRC, New.York.Structural.GenomiX.Research.Consortium.]]
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[[Category: Swaminathan, S.]]
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[[Category: PLP]]
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[[Category: new york structural genomix research consortium]]
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[[Category: nysgxrc]]
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[[Category: protein structure initiative]]
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[[Category: proteome]]
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[[Category: psi]]
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[[Category: pyridoxal phosphate]]
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[[Category: structural genomics]]
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[[Category: tim barrel]]
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[[Category: yeast hypothetical protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:22:11 2007''
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Current revision

CRYSTAL STRUCTURE OF A YEAST HYPOTHETICAL PROTEIN-A STRUCTURE FROM BNL'S HUMAN PROTEOME PROJECT

PDB ID 1b54

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