3cpi

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[[Image:3cpi.jpg|left|200px]]
 
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==Crystal structure of yeast Rab-GDI==
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The line below this paragraph, containing "STRUCTURE_3cpi", creates the "Structure Box" on the page.
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<StructureSection load='3cpi' size='340' side='right'caption='[[3cpi]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3cpi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CPI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cpi OCA], [https://pdbe.org/3cpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cpi RCSB], [https://www.ebi.ac.uk/pdbsum/3cpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cpi ProSAT]</span></td></tr>
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{{STRUCTURE_3cpi| PDB=3cpi | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GDI1_YEAST GDI1_YEAST] Regulates the GDP/GTP exchange reaction of SEC4 by inhibiting the dissociation of GDP from it, and the subsequent binding of GTP to SEC4. Plays an essential role in the yeast secretory pathway.<ref>PMID:8157010</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/3cpi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cpi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Rab GDP dissociation inhibitors (GDI)-facilitated extraction of prenylated Rab proteins from membranes plays an important role in vesicular membrane trafficking. The investigated thermodynamic properties of yeast Rab.GDI and Rab.MRS6 complexes demonstrated differences in the Rab binding properties of the closely related Rab GDI and MRS6 proteins, consistent with their functional diversity. The importance of the Rab C terminus and its prenylation for GDI/MRS6 binding was demonstrated using both biochemical and structural data. The presented structures of the apo-form yeast Rab GDI and its two complexes with unprenylated Rab proteins, together with the earlier published structures of the prenylated Ypt1.GDI, provide evidence of allosteric regulation of the GDI lipid binding site opening, which plays a key role in the proposed mechanism of GDI-mediated Rab extraction. We suggest a model for the interaction of GDI with prenylated Rab proteins that incorporates a stepwise increase in affinity as the three different partial interactions are successively formed.
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'''Crystal structure of yeast Rab-GDI'''
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A structural model of the GDP dissociation inhibitor rab membrane extraction mechanism.,Ignatev A, Kravchenko S, Rak A, Goody RS, Pylypenko O J Biol Chem. 2008 Jun 27;283(26):18377-84. Epub 2008 Apr 20. PMID:18426803<ref>PMID:18426803</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==Overview==
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</div>
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RabGDI-facilitated extraction of prenylated Rab proteins from membranes plays an important role in vesicular membrane trafficking. The investigated thermodynamic properties of yeast Rab:GDI and Rab:MRS6 complexes demonstrated differences in the Rab binding properties of the closely related RabGDI and MRS6 proteins, consistent with their functional diversity. The importance of the Rab C-terminus and its prenylation for GDI/MRS6 binding was demonstrated using both biochemical and structural data. The presented structures of the apo-form yeast RabGDI and its two complexes with unprenylated Rab proteins, together with the earlier published structures of the prenylated Ypt1:RabGDI provide evidence of allosteric regulation of the GDI lipid binding site opening, which plays a key role in the proposed mechanism of GDI mediated Rab extraction. We suggest a model for the interaction of GDI with prenylated Rab proteins which incorporates a stepwise increase in affinity as the 3 different partial interactions are successively formed.
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<div class="pdbe-citations 3cpi" style="background-color:#fffaf0;"></div>
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== References ==
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==About this Structure==
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<references/>
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3CPI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CPI OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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A structural model of the GDI rab membrane extraction mechanism., Ignatev A, Kravchenko S, Rak A, Goody RS, Pylypenko O, J Biol Chem. 2008 Apr 20;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18426803 18426803]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Goody RS]]
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[[Category: Goody, R S.]]
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[[Category: Ignatev A]]
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[[Category: Ignatev, A.]]
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[[Category: Kravchenko S]]
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[[Category: Kravchenko, S.]]
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[[Category: Pylypenko O]]
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[[Category: Pylypenko, O.]]
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[[Category: Rak A]]
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[[Category: Rak, A.]]
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[[Category: Cytoplasm]]
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[[Category: Gdi]]
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[[Category: Gtpase activation]]
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[[Category: Phosphoprotein]]
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[[Category: Protein transport]]
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[[Category: Rab]]
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[[Category: Vesicular transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:56:35 2008''
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Current revision

Crystal structure of yeast Rab-GDI

PDB ID 3cpi

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