2uun

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[[Image:2uun.jpg|left|200px]]
 
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==Crystal structure of C-phycocyanin from Phormidium, Lyngbya spp. (Marine) and Spirulina sp. (Fresh water) shows two different ways of energy transfer between two hexamers.==
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The line below this paragraph, containing "STRUCTURE_2uun", creates the "Structure Box" on the page.
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<StructureSection load='2uun' size='340' side='right'caption='[[2uun]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2uun]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptolyngbya_sp. Leptolyngbya sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
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{{STRUCTURE_2uun| PDB=2uun | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uun OCA], [https://pdbe.org/2uun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uun RCSB], [https://www.ebi.ac.uk/pdbsum/2uun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uun ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/2uun_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uun ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The present paper describes an efficient single step chromatographic method for purification of C-Phycocyanin from three cyanobacterial species, i.e., Spirulina sp. (freshwater), Phormidium sp. (marine water) and Lyngbya sp. (marine water). C-Phycocyanin from these cyanobacterial species was purified to homogeneity and some of their properties were investigated. The purification involves a multistep treatment of the crude extract by fractional precipitation with ammonium sulfate, followed by ion-exchange chromatography on DEAE-Sepharose CL-6B column. Pure C-Phycocyanin was finally obtained from Spirulina, Phormidium, and Lyngbya spp. with purity ratio (A620/A280) 4.42, 4.43, and 4.59, respectively, further the purity and homogeneity were confirmed by native and SDS-PAGE. The estimated molecular weights of purified C-PC from Spirulina, Phormidium, and Lyngbya spp. were 112, 131, and 81 kDa, respectively. SDS-PAGE of pure C-Phycocyanin yielded two bands corresponding to alpha and beta subunits. The results of SDS-PAGE demonstrate the same molecular weight of beta subunits (24.4 kDa) for all the three cyanobacterial species, whereas the molecular weight of the alpha subunit is different for all (17 kDa Spirulina sp., 19.1 kDa Phormidium sp., 15.2 kDa Lyngbya sp.). Thus, the C-Phycocyanin was characterized as (alphabeta)3 for Spirulina and Phormidium spp., while as (alphabeta)2 for Lyngbya sp.
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'''CRYSTAL STRUCTURE OF C-PHYCOCYANIN FROM PHORMIDIUM, LYNGBYA SPP. (MARINE) AND SPIRULINA SP. (FRESH WATER) SHOWS TWO DIFFERENT WAYS OF ENERGY TRANSFER BETWEEN TWO HEXAMERS.'''
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Purification and characterization of C-Phycocyanin from cyanobacterial species of marine and freshwater habitat.,Patel A, Mishra S, Pawar R, Ghosh PK Protein Expr Purif. 2005 Apr;40(2):248-55. PMID:015766866<ref>PMID:015766866</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2uun" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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C-phycocyanins from three cyanobacterial cultures of freshwater and marine habitat, Spirulina, Phormidium and Lyngbya spp., were purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Blue-coloured crystals in different crystal forms, monoclinic and hexagonal, were obtained for the three species. The crystals took 1-12 weeks to grow to full size using polyethylene glycols of different molecular weights as precipitants. The amino-acid sequences of these proteins show high similarity to other known C-phycocyanins from related organisms; however, the C-phycocyanins reported here showed different biochemical and biophysical properties, i.e. molecular weight, stability etc. The X-ray diffraction data were collected at resolutions of 3.0 A for the monoclinic and 3.2 and 3.6 A for the hexagonal forms. The unit-cell parameters corresponding to the monoclinic space group P2(1) are a = 107.33, b = 115.64, c = 183.26 A, beta = 90.03 degrees for Spirulina sp. C-phycocyanin and are similar for crystals of Phormidium and Lyngbya spp. C-phycocyanins. Crystals belonging to the hexagonal space group P6(3), with unit-cell parameters a = b = 154.97, c = 40.35 A and a = b = 151.96, c = 39.06 A, were also obtained for the C-phycocyanins from Spirulina and Lyngbya spp., respectively. The estimated solvent content is around 50% for the monoclinic crystals of all three species assuming the presence of two hexamers per asymmetric unit. The solvent content is 66.5 and 64.1% for the hexagonal crystals of C-phycocyanin from Spirulina and Lyngbya spp. assuming the presence of one alphabeta monomer per asymmetric unit.
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*[[Phycocyanin|Phycocyanin]]
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== References ==
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==About this Structure==
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<references/>
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2UUN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Leptolyngbya_sp. Leptolyngbya sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUN OCA].
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__TOC__
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</StructureSection>
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==Reference==
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[[Category: Large Structures]]
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X-ray crystallographic studies on C-phycocyanins from cyanobacteria from different habitats: marine and freshwater., Satyanarayana L, Suresh CG, Patel A, Mishra S, Ghosh PK, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Sep 1;61(Pt, 9):844-7. Epub 2005 Aug 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511175 16511175]
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[[Category: Leptolyngbya sp]]
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[[Category: Leptolyngbya sp.]]
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[[Category: K Ghosh P]]
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[[Category: Single protein]]
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[[Category: Mishra S]]
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[[Category: Ghosh, P K.]]
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[[Category: Patel A]]
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[[Category: Mishra, S.]]
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[[Category: Satyanarayana L]]
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[[Category: Patel, A.]]
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[[Category: Suresh CG]]
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[[Category: Satyanarayana, L.]]
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[[Category: Suresh, C G.]]
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[[Category: C-phycocyanin]]
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[[Category: Electron transport]]
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[[Category: Fresh water]]
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[[Category: Lyngbya sp]]
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[[Category: Marine]]
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[[Category: Phormidium]]
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[[Category: Spirulina sp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 14 11:22:54 2008''
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Current revision

Crystal structure of C-phycocyanin from Phormidium, Lyngbya spp. (Marine) and Spirulina sp. (Fresh water) shows two different ways of energy transfer between two hexamers.

PDB ID 2uun

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