1b7g

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(New page: 200px<br /><applet load="1b7g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b7g, resolution 2.05&Aring;" /> '''GLYCERALDEHYDE 3-PHO...)
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[[Image:1b7g.jpg|left|200px]]<br /><applet load="1b7g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1b7g, resolution 2.05&Aring;" />
 
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'''GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE'''<br />
 
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==Overview==
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==GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE==
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The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the, archaea shows low sequence identity (16-20%) with its eubacterial and, eukaryotic counterparts. The crystal structure of the apo GAPDH from, Sulfolobus solfataricus has been determined by multiple isomorphous, replacement at 2.05 A resolution. The enzyme has several differences in, secondary structure when compared with eubacterial GAPDHs, with an overall, increase in the number of alpha-helices. There is a relocation of the, active-site residues within the catalytic domain of the enzyme. The, thermostability of the S. solfataricus enzyme can be attributed to a, combination of an ion pair cluster and an intrasubunit disulphide bond.
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<StructureSection load='1b7g' size='340' side='right'caption='[[1b7g]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1b7g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B7G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b7g OCA], [https://pdbe.org/1b7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b7g RCSB], [https://www.ebi.ac.uk/pdbsum/1b7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b7g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/G3P_SACS2 G3P_SACS2] Can use both NAD and NADP as cofactors, but exhibits a marked preference for NADP.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b7/1b7g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b7g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the archaea shows low sequence identity (16-20%) with its eubacterial and eukaryotic counterparts. The crystal structure of the apo GAPDH from Sulfolobus solfataricus has been determined by multiple isomorphous replacement at 2.05 A resolution. The enzyme has several differences in secondary structure when compared with eubacterial GAPDHs, with an overall increase in the number of alpha-helices. There is a relocation of the active-site residues within the catalytic domain of the enzyme. The thermostability of the S. solfataricus enzyme can be attributed to a combination of an ion pair cluster and an intrasubunit disulphide bond.
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==About this Structure==
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Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus.,Isupov MN, Fleming TM, Dalby AR, Crowhurst GS, Bourne PC, Littlechild JA J Mol Biol. 1999 Aug 20;291(3):651-60. PMID:10448043<ref>PMID:10448043</ref>
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1B7G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.12 1.2.1.12] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B7G OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus., Isupov MN, Fleming TM, Dalby AR, Crowhurst GS, Bourne PC, Littlechild JA, J Mol Biol. 1999 Aug 20;291(3):651-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10448043 10448043]
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</div>
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[[Category: Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]]
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<div class="pdbe-citations 1b7g" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Isupov, M.N.]]
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[[Category: Littlechild, J.A.]]
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[[Category: SO4]]
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[[Category: archaea; hyperthermophile; gapdh; hyperthermophilic dehydrogenase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:25:42 2007''
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==See Also==
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*[[Aldehyde dehydrogenase 3D structures|Aldehyde dehydrogenase 3D structures]]
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*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Isupov MN]]
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[[Category: Littlechild JA]]

Current revision

GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE

PDB ID 1b7g

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