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| - | [[Image:2jxq.jpg|left|200px]] | |
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| - | <!--
| + | ==NMR structure of RNA duplex== |
| - | The line below this paragraph, containing "STRUCTURE_2jxq", creates the "Structure Box" on the page.
| + | <StructureSection load='2jxq' size='340' side='right'caption='[[2jxq]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2jxq]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JXQ FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxq OCA], [https://pdbe.org/2jxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jxq RCSB], [https://www.ebi.ac.uk/pdbsum/2jxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jxq ProSAT]</span></td></tr> |
| - | {{STRUCTURE_2jxq| PDB=2jxq | SCENE= }}
| + | </table> |
| - | | + | __TOC__ |
| - | '''NMR structure of RNA duplex'''
| + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | | + | [[Category: Adamiak RW]] |
| - | ==Overview== | + | [[Category: Gdaniec Z]] |
| - | The RNA single bulge motif is an unpaired residue within a strand of several complementary base pairs. To gain insight into structural changes induced by the presence of the adenosine bulge on RNA duplex, the solution structures of RNA duplex containing a single adenine bulge (5'-GCAGAAGAGCG-3'/5'-CGCUCUCUGC-3') and a reference duplex with all Watson-Crick base pairs (5'-GCAGAGAGCG-3'/5'-CGCUCUCUGC-3') have been determined by NMR spectroscopy. The reference duplex structure is a regular right-handed helix with all of the attributes of an A-type helix. In the bulged duplex, single adenine bulge stacks into the helix, and the bulge region forms a well-defined structure. Both structures were analyzed by the use of calculated helical parameters. Distortions induced by the accommodation of unpaired residue into the helical structure propagate over the entire structure and are manifested as the reduced base pairs inclination and x-displacement. Intrahelical position of bulged adenine A5 is stabilized by efficient stacking with 5'-neighboring residues G4.
| + | [[Category: Popenda L]] |
| - | | + | |
| - | ==About this Structure== | + | |
| - | 2JXQ is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JXQ OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | Bulged adenosine influence on the RNA duplex conformation in solution., Popenda L, Adamiak RW, Gdaniec Z, Biochemistry. 2008 May 6;47(18):5059-67. Epub 2008 Apr 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18399645 18399645]
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| - | [[Category: Protein complex]] | + | |
| - | [[Category: Adamiak, R W.]] | + | |
| - | [[Category: Gdaniec, Z.]] | + | |
| - | [[Category: Popenda, L.]] | + | |
| - | [[Category: A-type]] | + | |
| - | [[Category: Duplex]] | + | |
| - | [[Category: Rna]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 21:30:53 2008''
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