1b8u

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(New page: 200px<br /><applet load="1b8u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b8u, resolution 2.5&Aring;" /> '''MALATE DEHYDROGENASE ...)
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[[Image:1b8u.jpg|left|200px]]<br /><applet load="1b8u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1b8u, resolution 2.5&Aring;" />
 
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'''MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM'''<br />
 
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==Overview==
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==MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM==
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Aquaspillium arcticum is a psychrophilic bacterium that was isolated from, arctic sediment and grows optimally at 4 degrees C. We have cloned, purified, and characterized malate dehydrogenase from A. arcticum (Aa, MDH). We also have determined the crystal structures of apo-Aa MDH, Aa, MDH.NADH binary complex, and Aa MDH.NAD.oxaloacetate ternary complex at, 1.9-, 2.1-, and 2.5-A resolutions, respectively. The Aa MDH sequence is, most closely related to the sequence of a thermophilic MDH from Thermus, flavus (Tf MDH), showing 61% sequence identity and over 90% sequence, similarity. Stability studies show that Aa MDH has a half-life of 10 min, at 55 degrees C, whereas Tf MDH is fully active at 90 degrees C for 1 h., Aa MDH shows 2-3-fold higher catalytic efficiency compared with a, mesophilic or a thermophilic MDH at the temperature range 4-10 degrees C., Structural comparison of Aa MDH and Tf MDH suggests that the increased, relative flexibility of active site residues, favorable surface charge, distribution for substrate and cofactor, and the reduced intersubunit ion, pair interactions may be the major factors for the efficient catalytic, activity of Aa MDH at low temperatures.
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<StructureSection load='1b8u' size='340' side='right'caption='[[1b8u]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1b8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquaspirillum_arcticum Aquaspirillum arcticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B8U FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b8u OCA], [https://pdbe.org/1b8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b8u RCSB], [https://www.ebi.ac.uk/pdbsum/1b8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b8u ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MDH_AQUAR MDH_AQUAR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b8/1b8u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b8u ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1B8U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aquaspirillum_arcticum Aquaspirillum arcticum] with OAA and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B8U OCA].
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*[[Malate Dehydrogenase 3D structures|Malate Dehydrogenase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for cold adaptation. Sequence, biochemical properties, and crystal structure of malate dehydrogenase from a psychrophile Aquaspirillium arcticum., Kim SY, Hwang KY, Kim SH, Sung HC, Han YS, Cho Y, J Biol Chem. 1999 Apr 23;274(17):11761-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10206992 10206992]
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[[Category: Aquaspirillum arcticum]]
[[Category: Aquaspirillum arcticum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cho, Y.]]
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[[Category: Cho Y]]
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[[Category: Han, Y.S.]]
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[[Category: Han YS]]
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[[Category: Hwang, K.Y.]]
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[[Category: Hwang KY]]
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[[Category: Kim, S.H.]]
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[[Category: Kim S-H]]
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[[Category: Kim, S.Y.]]
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[[Category: Kim SY]]
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[[Category: NAD]]
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[[Category: OAA]]
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[[Category: dehydrogenase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:27:50 2007''
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Current revision

MALATE DEHYDROGENASE FROM AQUASPIRILLUM ARCTICUM

PDB ID 1b8u

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