3cc4

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[[Image:3cc4.jpg|left|200px]]
 
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==Co-crystal Structure of Anisomycin Bound to the 50S Ribosomal Subunit==
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The line below this paragraph, containing "STRUCTURE_3cc4", creates the "Structure Box" on the page.
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<StructureSection load='3cc4' size='340' side='right'caption='[[3cc4]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cc4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CC4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1MA:6-HYDRO-1-METHYLADENOSINE-5-MONOPHOSPHATE'>1MA</scene>, <scene name='pdbligand=ANM:ANISOMYCIN'>ANM</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene></td></tr>
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{{STRUCTURE_3cc4| PDB=3cc4 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cc4 OCA], [https://pdbe.org/3cc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cc4 RCSB], [https://www.ebi.ac.uk/pdbsum/3cc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cc4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL2_HALMA RL2_HALMA] One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome (By similarity).[HAMAP-Rule:MF_01320_A]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/3cc4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cc4 ConSurf].
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<div style="clear:both"></div>
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'''Co-crystal Structure of Anisomycin Bound to the 50S Ribosomal Subunit'''
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==See Also==
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Eleven mutations that make Haloarcula marismortui resistant to anisomycin, an antibiotic that competes with the amino acid side chains of aminoacyl tRNAs for binding to the A-site cleft of the large ribosomal unit, have been identified in 23S rRNA. The correlation observed between the sensitivity of H. marismortui to anisomycin and the affinity of its large ribosomal subunits for the drug indicates that its response to anisomycin is determined primarily by the binding of the drug to its large ribosomal subunit. The structures of large ribosomal subunits containing resistance mutations show that these mutations can be divided into two classes: (1) those that interfere with specific drug-ribosome interactions and (2) those that stabilize the apo conformation of the A-site cleft of the ribosome relative to its drug-bound conformation. The conformational effects of some mutations of the second kind propagate through the ribosome for considerable distances and are reversed when A-site substrates bind to the ribosome.
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==About this Structure==
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3CC4 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CC4 OCA].
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==Reference==
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Mutations outside the anisomycin-binding site can make ribosomes drug-resistant., Blaha G, Gurel G, Schroeder SJ, Moore PB, Steitz TA, J Mol Biol. 2008 Jun 6;379(3):505-19. Epub 2008 Apr 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18455733 18455733]
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[[Category: Haloarcula marismortui]]
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[[Category: Protein complex]]
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[[Category: Blaha, G.]]
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[[Category: Gurel, G.]]
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[[Category: Anisomycin]]
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[[Category: Co-crystal]]
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[[Category: Haloarcula marismortui]]
[[Category: Haloarcula marismortui]]
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[[Category: Large ribosomal subunit]]
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[[Category: Large Structures]]
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[[Category: Ribosome]]
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[[Category: Blaha G]]
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[[Category: Wild type]]
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[[Category: Gurel G]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 21:58:39 2008''
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Current revision

Co-crystal Structure of Anisomycin Bound to the 50S Ribosomal Subunit

PDB ID 3cc4

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