2fzv

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[[Image:2fzv.gif|left|200px]]
 
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==Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri==
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The line below this paragraph, containing "STRUCTURE_2fzv", creates the "Structure Box" on the page.
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<StructureSection load='2fzv' size='340' side='right'caption='[[2fzv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fzv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri_2a_str._2457T Shigella flexneri 2a str. 2457T]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FZV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_2fzv| PDB=2fzv | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fzv OCA], [https://pdbe.org/2fzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fzv RCSB], [https://www.ebi.ac.uk/pdbsum/2fzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fzv ProSAT], [https://www.topsan.org/Proteins/MCSG/2fzv TOPSAN]</span></td></tr>
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</table>
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'''Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri'''
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== Function ==
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[https://www.uniprot.org/uniprot/ARREH_SHIFL ARREH_SHIFL] Has NADPH-dependent FMN reductase activity and very low azoreductase activity. No activity with NADH.<ref>PMID:17962405</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The arsH gene or its homologs are a frequent part of the arsenic resistance system in bacteria and eukaryotes. Although a specific biological function of the gene product is unknown, the ArsH protein was annotated as a member of the NADPH-dependent FMN reductase family based on a conserved (T/S)XRXXSX(T/S) fingerprint motif common for FMN binding proteins. Presented here are the first crystal structure of an ArsH protein from Shigella flexneri refined at 1.7 A resolution and results of enzymatic activity assays that revealed a strong NADPH-dependent FMN reductase and low azoreductase activities. The ArsH apo protein has an alpha/beta/alpha-fold typical for FMN binding proteins. The asymmetric unit consists of four monomers, which form a tetramer. Buried surface analysis suggests that this tetramer is likely to be the relevant biological assembly. Dynamic light scattering experiments are consistent with this hypothesis and show that ArsH in solution at room temperature does exist predominantly in the tetrameric form.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fz/2fzv_consurf.spt"</scriptWhenChecked>
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2FZV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Shigella_flexneri_2a Shigella flexneri 2a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FZV OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of an apo form of Shigella flexneri ArsH protein with an NADPH-dependent FMN reductase activity., Vorontsov II, Minasov G, Brunzelle JS, Shuvalova L, Kiryukhina O, Collart FR, Anderson WF, Protein Sci. 2007 Nov;16(11):2483-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17962405 17962405]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fzv ConSurf].
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[[Category: Shigella flexneri 2a]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Anderson, W F.]]
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<references/>
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[[Category: Brunzelle, J S.]]
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__TOC__
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[[Category: Collart, F R.]]
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</StructureSection>
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Large Structures]]
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[[Category: Minasov, G.]]
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[[Category: Shigella flexneri 2a str. 2457T]]
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[[Category: Shuvalova, L.]]
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[[Category: Anderson WF]]
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[[Category: Vorontsov, I I.]]
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[[Category: Brunzelle JS]]
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[[Category: Crystal structure]]
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[[Category: Collart FR]]
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[[Category: Flavin binding protein]]
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[[Category: Joachimiak A]]
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[[Category: Mcsg]]
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[[Category: Minasov G]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Shuvalova L]]
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[[Category: Protein structure initiative]]
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[[Category: Vorontsov II]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:22:47 2008''
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Current revision

Crystal Structure of an apo form of a Flavin-binding Protein from Shigella flexneri

PDB ID 2fzv

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