3bs1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:30, 21 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:3bs1.jpg|left|200px]]
 
-
<!--
+
==Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with a Novel Mode of Binding==
-
The line below this paragraph, containing "STRUCTURE_3bs1", creates the "Structure Box" on the page.
+
<StructureSection load='3bs1' size='340' side='right'caption='[[3bs1]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3bs1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BS1 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRU:5-BROMO-2-DEOXYURIDINE-5-MONOPHOSPHATE'>BRU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
{{STRUCTURE_3bs1| PDB=3bs1 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bs1 OCA], [https://pdbe.org/3bs1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bs1 RCSB], [https://www.ebi.ac.uk/pdbsum/3bs1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bs1 ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with a Novel Mode of Binding'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/AGRA_STAAU AGRA_STAAU] Required for high-level post-exponential phase expression of a series of secreted proteins.
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The LytTR domain is a DNA-binding motif found within the AlgR/AgrA/LytR family of transcription factors that regulate virulence factor and toxin gene expression in pathogenic bacteria. This previously uncharacterized domain lacks sequence similarity with proteins of known structure. The crystal structure of the DNA-binding domain of Staphylococcus aureus AgrA complexed with a DNA pentadecamer duplex has been determined at 1.6 A resolution. The structure establishes a 10-stranded beta fold for the LytTR domain and reveals its mode of interaction with DNA. Residues within loop regions of AgrA contact two successive major grooves and the intervening minor groove on one face of the oligonucleotide duplex, inducing a substantial bend in the DNA. Loss of DNA binding upon substitution of key interacting residues in AgrA supports the observed binding mode. This mode of protein-DNA interaction provides a potential target for future antimicrobial drug design.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/3bs1_consurf.spt"</scriptWhenChecked>
-
3BS1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BS1 OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with an Unusual Mode of Binding., Sidote DJ, Barbieri CM, Wu T, Stock AM, Structure. 2008 May;16(5):727-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18462677 18462677]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bs1 ConSurf].
-
[[Category: Protein complex]]
+
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
-
[[Category: Barbieri, C.]]
+
[[Category: Barbieri C]]
-
[[Category: Sidote, D J.]]
+
[[Category: Sidote DJ]]
-
[[Category: Stock, A M.]]
+
[[Category: Stock AM]]
-
[[Category: Wu, T.]]
+
[[Category: Wu T]]
-
[[Category: Activator]]
+
-
[[Category: Agra]]
+
-
[[Category: Cytoplasm]]
+
-
[[Category: Dna binding domain]]
+
-
[[Category: Dna-binding]]
+
-
[[Category: Lyttr]]
+
-
[[Category: Phosphoprotein]]
+
-
[[Category: Response regulator]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transcription regulation]]
+
-
[[Category: Transcription regulator]]
+
-
[[Category: Two-component regulatory system]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:35:55 2008''
+

Current revision

Structure of the Staphylococcus aureus AgrA LytTR Domain Bound to DNA Reveals a Beta Fold with a Novel Mode of Binding

PDB ID 3bs1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3bs1"
Personal tools