1bcf

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(New page: 200px<br /><applet load="1bcf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bcf, resolution 2.9&Aring;" /> '''THE STRUCTURE OF A UN...)
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[[Image:1bcf.gif|left|200px]]<br /><applet load="1bcf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bcf, resolution 2.9&Aring;" />
 
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'''THE STRUCTURE OF A UNIQUE, TWO-FOLD SYMMETRIC, HAEM-BINDING SITE'''<br />
 
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==Overview==
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==THE STRUCTURE OF A UNIQUE, TWO-FOLD SYMMETRIC, HAEM-BINDING SITE==
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Bacterioferritin of Escherichia coli, also known as cytochrome b1, is a, hollow, nearly spherical shell made up of 24 identical protein subunits, and 12 haems. We have solved this structure in a tetragonal crystal form, at 2.9 A resolution. We find that each haem is bound in a pocket formed by, the interface between a pair of symmetry-related subunits. The, quasi-twofold axis of the haem is closely aligned with the local twofold, axis relating these subunits. The axial ligands of the haem are sulphurs, of two equivalent methionyl residues (Met 52) from the symmetry-related, subunits. A cluster of four water molecules is trapped in the gap between, the upper edge of the haem and two extended protein loops which close off, the haem from the outer aqueous environment. This is the first structure, of a bis-methionine ligated haem-binding site and the first case of a, twofold symmetric haem-binding site.
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<StructureSection load='1bcf' size='340' side='right'caption='[[1bcf]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bcf]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BCF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bcf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcf OCA], [https://pdbe.org/1bcf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bcf RCSB], [https://www.ebi.ac.uk/pdbsum/1bcf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BFR_ECOLI BFR_ECOLI] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex. The mineralized iron core can contain as many as 2700 iron atoms/24-meric molecule.<ref>PMID:10769150</ref> <ref>PMID:14636073</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/1bcf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bcf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BCF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN and HEM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BCF OCA].
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*[[Ferritin 3D structures|Ferritin 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structure of a unique twofold symmetric haem-binding site., Frolow F, Kalb AJ, Yariv J, Nat Struct Biol. 1994 Jul;1(7):453-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7664064 7664064]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Frolow, F.]]
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[[Category: Frolow F]]
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[[Category: Gilboa, A.J.Kalb.]]
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[[Category: Yariv J]]
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[[Category: Yariv, J.]]
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[[Category: HEM]]
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[[Category: MN]]
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[[Category: iron storage and electron transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:32:27 2007''
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Current revision

THE STRUCTURE OF A UNIQUE, TWO-FOLD SYMMETRIC, HAEM-BINDING SITE

PDB ID 1bcf

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