2pi3

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[[Image:2pi3.jpg|left|200px]]
 
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==The crystal structure of OspA mutant==
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The line below this paragraph, containing "STRUCTURE_2pi3", creates the "Structure Box" on the page.
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<StructureSection load='2pi3' size='340' side='right'caption='[[2pi3]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pi3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Borreliella_burgdorferi Borreliella burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PI3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pi3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pi3 OCA], [https://pdbe.org/2pi3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pi3 RCSB], [https://www.ebi.ac.uk/pdbsum/2pi3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pi3 ProSAT]</span></td></tr>
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{{STRUCTURE_2pi3| PDB=2pi3 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q45040_BORBG Q45040_BORBG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/2pi3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pi3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Site-directed mutagenesis is a powerful tool for altering the structure and function of proteins in a focused manner. Here, we examined how a model beta-sheet protein could be tuned by mutation of numerous surface-exposed residues to aromatic amino acids. We designed these aromatic side chain "clusters" at highly solvent-exposed positions in the flat, single-layer beta-sheet of Borrelia outer surface protein A (OspA). This unusual beta-sheet scaffold allows us to interrogate the effects of these mutations in the context of well-defined structure but in the absence of the strong scaffolding effects of globular protein architecture. We anticipated that the introduction of a cluster of aromatic amino acid residues on the beta-sheet surface would result in large conformational changes and/or stabilization and thereby provide new means of controlling the properties of beta-sheets. Surprisingly, X-ray crystal structures revealed that the introduction of aromatic clusters produced only subtle conformational changes in the OspA beta-sheet. Additionally, despite burying a large degree of hydrophobic surface area, the aromatic cluster mutants were slightly less stable than the wild-type scaffold. These results thereby demonstrate that the introduction of aromatic cluster mutations can serve as a means for subtly modulating beta-sheet conformation in protein design.
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'''The crystal structure of OspA mutant'''
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Aromatic cluster mutations produce focal modulations of beta-sheet structure.,Biancalana M, Makabe K, Yan S, Koide S Protein Sci. 2015 May;24(5):841-9. doi: 10.1002/pro.2657. Epub 2015 Mar 25. PMID:25645104<ref>PMID:25645104</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2pi3" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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2PI3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI3 OCA].
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*[[Outer surface protein|Outer surface protein]]
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[[Category: Borrelia burgdorferi]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Biancalana, M.]]
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__TOC__
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[[Category: Koide, S.]]
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</StructureSection>
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[[Category: Makabe, K.]]
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[[Category: Borreliella burgdorferi]]
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[[Category: Terechko, V.]]
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[[Category: Large Structures]]
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[[Category: Beta sheet]]
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[[Category: Biancalana M]]
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[[Category: Membrane protein]]
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[[Category: Koide S]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:13:04 2008''
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[[Category: Makabe K]]
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[[Category: Terechko V]]

Current revision

The crystal structure of OspA mutant

PDB ID 2pi3

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