1bcx

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(New page: 200px<br /><applet load="1bcx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bcx, resolution 1.81&Aring;" /> '''MUTATIONAL AND CRYST...)
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[[Image:1bcx.jpg|left|200px]]<br /><applet load="1bcx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bcx, resolution 1.81&Aring;" />
 
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'''MUTATIONAL AND CRYSTALLOGRAPHIC ANALYSES OF THE ACTIVE SITE RESIDUES OF THE BACILLUS CIRCULANS XYLANASE'''<br />
 
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==Overview==
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==MUTATIONAL AND CRYSTALLOGRAPHIC ANALYSES OF THE ACTIVE SITE RESIDUES OF THE BACILLUS CIRCULANS XYLANASE==
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Using site-directed mutagenesis we have investigated the catalytic, residues in a xylanase from Bacillus circulans. Analysis of the mutants, E78D and E172D indicated that mutations in these conserved residues do not, grossly alter the structure of the enzyme and that these residues, participate in the catalytic mechanism. We have now determined the crystal, structure of an enzyme-substrate complex to 108 A resolution using a, catalytically incompetent mutant (E172C). In addition to the catalytic, residues, Glu 78 and Glu 172, we have identified 2 tyrosine residues, Tyr, 69 and Tyr 80, which likely function in substrate binding, and an arginine, residue, Arg 112, which plays an important role in the active site of this, enzyme. On the basis of our work we would propose that Glu 78 is the, nucleophile and that Glu 172 is the acid-base catalyst in the reaction.
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<StructureSection load='1bcx' size='340' side='right'caption='[[1bcx]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bcx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BCX FirstGlance]. <br>
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1BCX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BCX OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PRD_900116:4beta-beta-xylobiose'>PRD_900116</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcx OCA], [https://pdbe.org/1bcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bcx RCSB], [https://www.ebi.ac.uk/pdbsum/1bcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bcx ProSAT]</span></td></tr>
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Mutational and crystallographic analyses of the active site residues of the Bacillus circulans xylanase., Wakarchuk WW, Campbell RL, Sung WL, Davoodi J, Yaguchi M, Protein Sci. 1994 Mar;3(3):467-75. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8019418 8019418]
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</table>
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[[Category: Bacillus circulans]]
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== Function ==
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[[Category: Endo-1,4-beta-xylanase]]
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[https://www.uniprot.org/uniprot/XYNA_NIACI XYNA_NIACI]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Campbell, R.L.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Wakarchuk, W.W.]]
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Check<jmol>
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[[Category: SO4]]
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<jmolCheckbox>
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[[Category: hydrolase(xylan degradation)]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bc/1bcx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:33:11 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bcx ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Niallia circulans]]
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[[Category: Campbell RL]]
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[[Category: Wakarchuk WW]]

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MUTATIONAL AND CRYSTALLOGRAPHIC ANALYSES OF THE ACTIVE SITE RESIDUES OF THE BACILLUS CIRCULANS XYLANASE

PDB ID 1bcx

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