3ci3

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[[Image:3ci3.jpg|left|200px]]
 
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==Structure of the PduO-type ATP:co(I)rrinoid adenosyltransferase from Lactobacillus reuteri complexed with partial adenosylcobalamin and PPPi==
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The line below this paragraph, containing "STRUCTURE_3ci3", creates the "Structure Box" on the page.
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<StructureSection load='3ci3' size='340' side='right'caption='[[3ci3]], [[Resolution|resolution]] 1.11&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3ci3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri Limosilactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CI3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.11&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PO:TRIPHOSPHATE'>3PO</scene>, <scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=B12:COBALAMIN'>B12</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3ci3| PDB=3ci3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ci3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ci3 OCA], [https://pdbe.org/3ci3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ci3 RCSB], [https://www.ebi.ac.uk/pdbsum/3ci3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ci3 ProSAT]</span></td></tr>
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</table>
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'''Structure of the PduO-type ATP:co(I)rrinoid adenosyltransferase from Lactobacillus reuteri complexed with partial adenosylcobalamin and PPPi'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q50EJ2_LIMRT Q50EJ2_LIMRT]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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ATP:cob(I)alamin adenosyltransferases (ACAs) catalyze the transfer of the 5'-deoxyadenosyl moiety from ATP to the upper axial ligand position of cobalamin in the synthesis of coenzyme B 12. For the ACA-catalyzed reaction to proceed, cob(II)alamin must be reduced to cob(I)alamin in the enzyme active site. This reduction is facilitated through the generation of a four-coordinate cob(II)alamin intermediate on the enzyme. We have determined the high-resolution crystal structure of a human-type ACA from Lactobacillus reuteri with a four-coordinate cob(II)alamin bound in the enzyme active site and with the product, adenosylcobalamin, partially occupied in the active site. The assembled structures represent snapshots of the steps in the ACA-catalyzed formation of the cobalt-carbon bond of coenzyme B 12. The structures define the corrinoid binding site and provide visual evidence for a base-off, four-coordinate cob(II)alamin intermediate. The complete structural description of ACA-mediated catalysis reveals the molecular features of four-coordinate cob(II)alamin stabilization and provides additional insights into the molecular basis for dysfunction in human patients suffering from methylmalonic aciduria.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/3ci3_consurf.spt"</scriptWhenChecked>
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3CI3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lactobacillus_reuteri Lactobacillus reuteri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CI3 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B12 is synthesized through a four-coordinate intermediate., St Maurice M, Mera P, Park K, Brunold TC, Escalante-Semerena JC, Rayment I, Biochemistry. 2008 May 27;47(21):5755-66. Epub 2008 May 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18452306 18452306]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ci3 ConSurf].
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[[Category: Lactobacillus reuteri]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Escalante-Semerena, J C.]]
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</StructureSection>
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[[Category: Maurice, M St.]]
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[[Category: Large Structures]]
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[[Category: Mera, P E.]]
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[[Category: Limosilactobacillus reuteri]]
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[[Category: Rayment, I.]]
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[[Category: Escalante-Semerena JC]]
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[[Category: Adenosylcobalamin binding]]
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[[Category: Maurice MSt]]
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[[Category: Adenosyltransferase variant]]
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[[Category: Mera PE]]
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[[Category: Atp binding]]
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[[Category: Rayment I]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:22:23 2008''
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Current revision

Structure of the PduO-type ATP:co(I)rrinoid adenosyltransferase from Lactobacillus reuteri complexed with partial adenosylcobalamin and PPPi

PDB ID 3ci3

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