1bhc

(Difference between revisions)

Current revision

BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE

Structural highlights

1bhc is a 10 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BPT1_BOVIN Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of a monoclinic form of bovine pancreatic trypsin inhibitor (BPTI) crystallized from a thiocyanate solution has been determined and refined at 2.7 A resolution. The space group is P21 with a = 71.56, b = 73.83, c = 64.47 A, beta = 93.9 degrees and Z = 20. The ten independent molecules were located by a multi-body molecular-replacement search as developed in the AMoRe program, starting from a single monomer model (PDB code: 6PTI). The molecular arrangement of the subunits is a decamer resulting from the combination of two orthogonal fivefold and twofold non-crystallographic axes. This builds a globular micelle-like particle which minimizes hydrophobic interactions with the solvent. The refinement was conducted with non-crystallographic symmetry constraints up to a final residual of R = 0.20 (Rfree= 0.26). The root-mean-square deviations from ideal geometry were 0.015 A and 1.6 degrees on bond distances and bond angles, respectively. Several sites for thiocyanate ions were analyzed.

The decameric structure of bovine pancreatic trypsin inhibitor (BPTI) crystallized from thiocyanate at 2.7 A resolution.,Hamiaux C, Prange T, Ries-Kautt M, Ducruix A, Lafont S, Astier JP, Veesler S Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):103-13. Epub 1999, Jan 1. PMID:10089400^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hamiaux C, Prange T, Ries-Kautt M, Ducruix A, Lafont S, Astier JP, Veesler S. The decameric structure of bovine pancreatic trypsin inhibitor (BPTI) crystallized from thiocyanate at 2.7 A resolution. Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):103-13. Epub 1999, Jan 1. PMID:10089400 doi:10.1107/S0907444998008725

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bhc"

@@ Line 1: / Line 1: @@
-[[Image:1bhc.jpg|left|200px]]<br /><applet load="1bhc" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1bhc, resolution 2.7&Aring;" />
-'''BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE'''<br />
-==Overview==
+==BOVINE PANCREATIC TRYPSIN INHIBITOR CRYSTALLIZED FROM THIOCYANATE==
-The structure of a monoclinic form of bovine pancreatic trypsin inhibitor, (BPTI) crystallized from a thiocyanate solution has been determined and, refined at 2.7 A resolution. The space group is P21 with a = 71.56, b =, 73.83, c = 64.47 A, beta = 93.9 degrees and Z = 20. The ten independent, molecules were located by a multi-body molecular-replacement search as, developed in the AMoRe program, starting from a single monomer model (PDB, code: 6PTI). The molecular arrangement of the subunits is a decamer, resulting from the combination of two orthogonal fivefold and twofold, non-crystallographic axes. This builds a globular micelle-like particle, which minimizes hydrophobic interactions with the solvent. The refinement, was conducted with non-crystallographic symmetry constraints up to a final, residual of R = 0.20 (Rfree= 0.26). The root-mean-square deviations from, ideal geometry were 0.015 A and 1.6 degrees on bond distances and bond, angles, respectively. Several sites for thiocyanate ions were analyzed.
+<StructureSection load='1bhc' size='340' side='right'caption='[[1bhc]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1bhc]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BHC FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhc OCA], [https://pdbe.org/1bhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bhc RCSB], [https://www.ebi.ac.uk/pdbsum/1bhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bhc ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/1bhc_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bhc ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The structure of a monoclinic form of bovine pancreatic trypsin inhibitor (BPTI) crystallized from a thiocyanate solution has been determined and refined at 2.7 A resolution. The space group is P21 with a = 71.56, b = 73.83, c = 64.47 A, beta = 93.9 degrees and Z = 20. The ten independent molecules were located by a multi-body molecular-replacement search as developed in the AMoRe program, starting from a single monomer model (PDB code: 6PTI). The molecular arrangement of the subunits is a decamer resulting from the combination of two orthogonal fivefold and twofold non-crystallographic axes. This builds a globular micelle-like particle which minimizes hydrophobic interactions with the solvent. The refinement was conducted with non-crystallographic symmetry constraints up to a final residual of R = 0.20 (Rfree= 0.26). The root-mean-square deviations from ideal geometry were 0.015 A and 1.6 degrees on bond distances and bond angles, respectively. Several sites for thiocyanate ions were analyzed.
-==About this Structure==
+The decameric structure of bovine pancreatic trypsin inhibitor (BPTI) crystallized from thiocyanate at 2.7 A resolution.,Hamiaux C, Prange T, Ries-Kautt M, Ducruix A, Lafont S, Astier JP, Veesler S Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):103-13. Epub 1999, Jan 1. PMID:10089400<ref>PMID:10089400</ref>
-BHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with SCN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BHC OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-The decameric structure of bovine pancreatic trypsin inhibitor (BPTI) crystallized from thiocyanate at 2.7 A resolution., Hamiaux C, Prange T, Ries-Kautt M, Ducruix A, Lafont S, Astier JP, Veesler S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):103-13. Epub 1999, Jan 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089400 10089400]
+</div>
-[[Category: Bos taurus]]
+<div class="pdbe-citations 1bhc" style="background-color:#fffaf0;"></div>
-[[Category: Single protein]]
-[[Category: Hamiaux, C.]]
-[[Category: Prange, T.]]
-[[Category: SCN]]
-[[Category: protease inhibitor]]
-[[Category: trypsin]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:39:34 2007''
+==See Also==
+*[[BPTI 3D structures|BPTI 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bos taurus]]
+[[Category: Large Structures]]
+[[Category: Hamiaux C]]
+[[Category: Prange T]]

Proteopedia	Main Page \| About \| Help \| FAQ \| Special pages \| Log in
	Categories: Bos taurus \| Large Structures \| Hamiaux C \| Prange T Printable version \| Disclaimers \| Privacy policy \| Current revision