1bjf

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(New page: 200px<br /><applet load="1bjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bjf, resolution 2.4&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1bjf.jpg|left|200px]]<br /><applet load="1bjf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bjf, resolution 2.4&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS==
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The crystal structure of calcium-bound unmyristoylated bovine neurocalcin, from Escherichia coli has been determined at 2.4 A resolution. The, three-dimensional structure reveals a highly compact structure consisting, of: (i) two pairs of calcium-binding EF-hands (EF1-EF2 and EF3-EF4); (ii), a calcium ion bound at EF2, EF3 and EF4 sites; and (iii) an EF1-hand that, is disabled from calcium-binding due to a Cys-Pro sequence in the, Ca2+-binding loop. The crystal structure of neurocalcin resembles, photoreceptor recoverin in overall topology, however its EF2- and, EF4-hands differ. Recently, neurocalcin in the calcium-bound state has, been shown to stimulate mammalian rod outer segment membrane guanylate, cyclase. A possible site for cyclase activity based on the, three-dimensional structure is discussed.
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<StructureSection load='1bjf' size='340' side='right'caption='[[1bjf]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bjf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BJF FirstGlance]. <br>
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1BJF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BJF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bjf OCA], [https://pdbe.org/1bjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bjf RCSB], [https://www.ebi.ac.uk/pdbsum/1bjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bjf ProSAT]</span></td></tr>
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Crystal structure of recombinant bovine neurocalcin., Vijay-Kumar S, Kumar VD, Nat Struct Biol. 1999 Jan;6(1):80-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9886296 9886296]
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</table>
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[[Category: Escherichia coli]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/NCALD_BOVIN NCALD_BOVIN] May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions.
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[[Category: Kumar, V.D.]]
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== Evolutionary Conservation ==
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[[Category: Vijay-Kumar, S.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: CA]]
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Check<jmol>
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[[Category: calcium-binding]]
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<jmolCheckbox>
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[[Category: myristoylation]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/1bjf_consurf.spt"</scriptWhenChecked>
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[[Category: neuronal specific guanylate cyclase activator]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:41:47 2007''
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bjf ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Kumar VD]]
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[[Category: Vijay-Kumar S]]

Current revision

CRYSTAL STRUCTURE OF RECOMBINANT BOVINE NEUROCALCIN DELTA AT 2.4 ANGSTROMS

PDB ID 1bjf

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