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1bkb

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(New page: 200px<br /><applet load="1bkb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bkb, resolution 1.75&Aring;" /> '''INITIATION FACTOR 5A...)
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[[Image:1bkb.jpg|left|200px]]<br /><applet load="1bkb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bkb, resolution 1.75&Aring;" />
 
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'''INITIATION FACTOR 5A FROM ARCHEBACTERIUM PYROBACULUM AEROPHILUM'''<br />
 
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==Overview==
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==INITIATION FACTOR 5A FROM ARCHEBACTERIUM PYROBACULUM AEROPHILUM==
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BACKGROUND: Translation initiation factor 5A (IF-5A) is reported to be, involved in the first step of peptide bond formation in translation, to be, involved in cell-cycle regulation and to be a cofactor for the Rev and Rex, transactivator proteins of human immunodeficiency virus-1 and T-cell, leukemia virus I, respectively. IF-5A contains an unusual amino acid, hypusine (N-epsilon-(4-aminobutyl-2-hydroxy)lysine), that is required for, its function. The first step in the post-translational modification of, lysine to hypusine is catalyzed by the enzyme deoxyhypusine synthase, the, structure of which has been published recently. RESULTS: IF-5A from the, archebacterium Pyrobaculum aerophilum has been heterologously expressed in, Escherichia coli with selenomethionine substitution. The crystal structure, of IF-5A has been determined by multiwavelength anomalous diffraction and, refined to 1.75 A. Unmodified P. aerophilum IF-5A is found to be a beta, structure with two domains and three separate hydrophobic cores., CONCLUSIONS: The lysine (Lys42) that is post-translationally modified by, deoxyhypusine synthase is found at one end of the IF-5A molecule in an, turn between beta strands beta4 and beta5; this lysine residue is freely, solvent accessible. The C-terminal domain is found to be homologous to the, cold-shock protein CspA of E. coli, which has a well characterized, RNA-binding fold, suggesting that IF-5A is involved in RNA binding.
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<StructureSection load='1bkb' size='340' side='right'caption='[[1bkb]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bkb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BKB FirstGlance]. <br>
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1BKB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BKB OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bkb OCA], [https://pdbe.org/1bkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bkb RCSB], [https://www.ebi.ac.uk/pdbsum/1bkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bkb ProSAT]</span></td></tr>
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Structure of translation initiation factor 5A from Pyrobaculum aerophilum at 1.75 A resolution., Peat TS, Newman J, Waldo GS, Berendzen J, Terwilliger TC, Structure. 1998 Sep 15;6(9):1207-14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9753699 9753699]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IF5A_PYRAE IF5A_PYRAE] Functions by promoting the formation of the first peptide bond.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/1bkb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bkb ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pyrobaculum aerophilum]]
[[Category: Pyrobaculum aerophilum]]
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[[Category: Single protein]]
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[[Category: Berendzen J]]
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[[Category: Berendzen, J.]]
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[[Category: Newman J]]
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[[Category: Newman, J.]]
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[[Category: Peat TS]]
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[[Category: Peat, T.S.]]
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[[Category: Terwilliger TC]]
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[[Category: Terwilliger, T.C.]]
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[[Category: Waldo GS]]
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[[Category: Waldo, G.S.]]
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[[Category: translation initiation factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:43:14 2007''
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Current revision

INITIATION FACTOR 5A FROM ARCHEBACTERIUM PYROBACULUM AEROPHILUM

PDB ID 1bkb

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