1bol

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(New page: 200px<br /><applet load="1bol" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bol, resolution 2.00&Aring;" /> '''THE CRYSTAL STRUCTUR...)
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[[Image:1bol.gif|left|200px]]<br /><applet load="1bol" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bol, resolution 2.00&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF RIBONUCLEASE RH FROM RHIZOPUS NIVEUS AT 2.0 A RESOLUTION'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF RIBONUCLEASE RH FROM RHIZOPUS NIVEUS AT 2.0 A RESOLUTION==
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The three-dimensional structure of ribonuclease Rh (RNase Rh), a new class, of microbial ribonuclease from Rhizopus niveus, has been determined at 2.0, A resolution. The overall structure of RNase Rh is completely different, from those of other previously studied RNases, such as RNase A from bovine, pancreas and RNase T1 from Aspergillus oryzae. In the structure of RNase, Rh, two histidine residues (His46 and His109) and one glutamic acid, residue (Glu105), which were predicted to be critical to the activity from, the chemical modification and mutagenesis experiments, are found to be, located close together, constructing the active site. The indole ring of, Trp49 plays an important role in preserving the active site structure by, its stacking interactions with the imidazole ring of His 109, and by, hydrogen bonding with the carboxyl group of Glu105. There exists a, hydrophobic pocket around the active site, which contains the aromatic, side-chain of Trp49 and Tyr57. The results of mutagenesis studies suggest, that this pocket is the base binding site of the substrate.
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<StructureSection load='1bol' size='340' side='right'caption='[[1bol]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bol]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizopus_niveus Rhizopus niveus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bol OCA], [https://pdbe.org/1bol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bol RCSB], [https://www.ebi.ac.uk/pdbsum/1bol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bol ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNRH_RHINI RNRH_RHINI] This is a base non-specific ribonuclease.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bol_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bol ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BOL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizopus_niveus Rhizopus niveus]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOL OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The crystal structure of ribonuclease Rh from Rhizopus niveus at 2.0 A resolution., Kurihara H, Nonaka T, Mitsui Y, Ohgi K, Irie M, Nakamura KT, J Mol Biol. 1996 Jan 19;255(2):310-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8551522 8551522]
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[[Category: Large Structures]]
[[Category: Rhizopus niveus]]
[[Category: Rhizopus niveus]]
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[[Category: Ribonuclease T(2)]]
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[[Category: Kurihara H]]
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[[Category: Single protein]]
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[[Category: Nakamura KT]]
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[[Category: Kurihara, H.]]
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[[Category: Nakamura, K.T.]]
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[[Category: ribonucleases]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:47:38 2007''
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Current revision

THE CRYSTAL STRUCTURE OF RIBONUCLEASE RH FROM RHIZOPUS NIVEUS AT 2.0 A RESOLUTION

PDB ID 1bol

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