1bos

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(New page: 200px<br /><applet load="1bos" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bos, resolution 2.8&Aring;" /> '''SHIGA-LIKE TOXIN COMP...)
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[[Image:1bos.gif|left|200px]]<br /><applet load="1bos" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bos, resolution 2.8&Aring;" />
 
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'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR'''<br />
 
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==Overview==
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==SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR==
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Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli, strains that cause disease in humans. Like other members of the Shiga, toxin family, it consists of an enzymatic (A) subunit and five copies of a, binding subunit (the B-pentamer). The B-pentamer binds to a specific, glycolipid, globotriaosylceramide (Gb3), on the surface of target cells, and thereby plays a crucial role in the entry of the toxin. Here we, present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer, complexed with an analogue of the Gb3 trisaccharide. The structure reveals, a surprising density of binding sites, with three trisaccharide molecules, bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind, to one side of the B-pentamer, providing further evidence that this side, faces the cell membrane. The structural model is consistent with data from, site-directed mutagenesis and binding of carbohydrate analogues, and, allows the rational design of therapeutic Gb3 analogues that block the, attachment of toxin to cells.
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<StructureSection load='1bos' size='340' side='right'caption='[[1bos]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bos]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_H-19B Enterobacteria phage H-19B] and [https://en.wikipedia.org/wiki/Phage_h30 Phage h30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [https://pdbe.org/1bos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB], [https://www.ebi.ac.uk/pdbsum/1bos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bos ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bos_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bos ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
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==About this Structure==
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Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3.,Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303<ref>PMID:9485303</ref>
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1BOS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_h30,_bacteriophage_h19b Bacteriophage h30, bacteriophage h19b] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9485303 9485303]
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</div>
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[[Category: Bacteriophage h30, bacteriophage h19b]]
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<div class="pdbe-citations 1bos" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Armstrong, G.D.]]
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[[Category: Boodhoo, A.]]
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[[Category: Brunton, J.L.]]
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[[Category: Cummings, M.D.]]
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[[Category: Hazes, B.]]
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[[Category: Ling, H.]]
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[[Category: Read, R.J.]]
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[[Category: GAL]]
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[[Category: ob-fold]]
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[[Category: protein-carbohydrate recognition]]
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[[Category: receptor binding]]
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[[Category: toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:47:58 2007''
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==See Also==
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*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Enterobacteria phage H-19B]]
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[[Category: Large Structures]]
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[[Category: Phage h30]]
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[[Category: Armstrong GD]]
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[[Category: Boodhoo A]]
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[[Category: Brunton JL]]
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[[Category: Cummings MD]]
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[[Category: Hazes B]]
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[[Category: Ling H]]
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[[Category: Read RJ]]

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SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR

PDB ID 1bos

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