3cio

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[[Image:3cio.jpg|left|200px]]
 
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==The kinase domain of Escherichia coli tyrosine kinase ETK==
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The line below this paragraph, containing "STRUCTURE_3cio", creates the "Structure Box" on the page.
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<StructureSection load='3cio' size='340' side='right'caption='[[3cio]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3cio]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CIO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_3cio| PDB=3cio | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cio OCA], [https://pdbe.org/3cio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cio RCSB], [https://www.ebi.ac.uk/pdbsum/3cio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cio ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ETK_ECOLI ETK_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/3cio_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cio ConSurf].
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<div style="clear:both"></div>
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'''The kinase domain of Escherichia coli tyrosine kinase ETK'''
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==See Also==
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*[[Tyrosine kinase 3D structures|Tyrosine kinase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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While protein tyrosine (Tyr) kinases (PTKs) have been extensively characterized in eukaryotes, far less is known about their emerging counterparts in prokaryotes. The inner-membrane Wzc/Etk protein belongs to the bacterial PTK family, which has an important function in regulating the polymerization and transport of virulence-determining capsular polysaccharide (CPS). The kinase uses a unique two-step activation process involving intra-phosphorylation of a Tyr residue, although the molecular mechanism remains unknown. Herein, we report the first crystal structure of a bacterial PTK, the C-terminal kinase domain of Escherichia coli Tyr kinase (Etk) at 2.5-A resolution. The fold of the Etk kinase domain differs markedly from that of eukaryotic PTKs. Based on the observed structure and supporting mass spectrometric evidence of Etk, a unique activation mechanism is proposed that involves the phosphorylated Tyr residue, Y574, at the active site and its specific interaction with a previously unidentified key Arg residue, R614, to unblock the active site. Both in vitro kinase activity and in vivo antibiotics resistance studies using structure-guided mutants further support the novel activation mechanism.
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Jia Z]]
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3CIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CIO OCA].
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[[Category: Lee DC]]
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[[Category: Zheng J]]
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==Reference==
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Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism., Lee DC, Zheng J, She YM, Jia Z, EMBO J. 2008 May 22;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18497741 18497741]
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[[Category: Escherichia coli]]
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[[Category: Non-specific protein-tyrosine kinase]]
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[[Category: Single protein]]
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[[Category: Jia, Z.]]
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[[Category: Lee, D C.]]
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[[Category: Zheng, J.]]
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[[Category: Escherichia coli tyrosine kinase domain]]
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[[Category: Etk]]
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[[Category: Inner membrane]]
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[[Category: Membrane]]
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[[Category: Phosphoprotein]]
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[[Category: Signaling protein]]
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[[Category: Transferase]]
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[[Category: Transmembrane]]
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[[Category: Tyrosine-protein kinase]]
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[[Category: Wzc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:55:14 2008''
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Current revision

The kinase domain of Escherichia coli tyrosine kinase ETK

PDB ID 3cio

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