2z69

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[[Image:2z69.jpg|left|200px]]
 
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==Crystal Structure of the sensor domain of the transcriptional regulator DNR from Pseudomonas aeruginosa==
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The line below this paragraph, containing "STRUCTURE_2z69", creates the "Structure Box" on the page.
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<StructureSection load='2z69' size='340' side='right'caption='[[2z69]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2z69]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z69 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z69 OCA], [https://pdbe.org/2z69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z69 RCSB], [https://www.ebi.ac.uk/pdbsum/2z69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z69 ProSAT]</span></td></tr>
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{{STRUCTURE_2z69| PDB=2z69 | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of the sensor domain of the transcriptional regulator DNR from Pseudomonas aeruginosa'''
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[https://www.uniprot.org/uniprot/Q51441_PSEAI Q51441_PSEAI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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All denitrifying bacteria can keep the steady-state concentrations of nitrite and nitric oxide (NO) below cytotoxic levels, controlling the expression of the denitrification gene clusters by redox signaling, mainly through transcriptional regulators belonging either to the DNR (dissimilative nitrate respiration regulator) or to the NnrR (nitrite and nitric oxide reductase regulator) subgroups of the FNR (fumarate and nitrate reductase regulatory protein)-CRP (cAMP receptor protein) superfamily. The NO dependence of the transcriptional activity of promoters regulated by these transcription factors has suggested that they may act as NO sensors in vivo. Despite great interest in the regulation of denitrification, which in Pseudomonas aeruginosa is strictly related to virulence, functional and structural characterization of these NO sensors is still lacking. Here we present the three-dimensional structure of the sensor domain of the DNR from P. aeruginosa at 2.1 A resolution. This is the first structure of a putative NO-sensing bacterial transcriptional regulator and reveals the presence of a large hydrophobic cavity that may be the cofactor binding site. Parallel spectroscopic evidence indicates that apo-DNR binds heme in vitro and that the heme-bound form reacts with carbon monoxide and NO, thus supporting the hypothesis that NO sensing involves gas binding to the ferrous heme. Preliminary experiments indicate that heterologous expression of the heme-containing DNR yields a protein able to bind DNA in vitro.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z69_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2Z69 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z69 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z69 ConSurf].
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NO sensing in Pseudomonas aeruginosa: structure of the transcriptional regulator DNR., Giardina G, Rinaldo S, Johnson KA, Di Matteo A, Brunori M, Cutruzzola F, J Mol Biol. 2008 May 16;378(5):1002-15. Epub 2008 Mar 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18420222 18420222]
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<div style="clear:both"></div>
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[[Category: Pseudomonas aeruginosa]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Giardina, G.]]
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[[Category: Large Structures]]
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[[Category: Johnson, K A.]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Matteo, A Di.]]
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[[Category: Di Matteo A]]
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[[Category: Beta barrel]]
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[[Category: Giardina G]]
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[[Category: Dimerization helix]]
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[[Category: Johnson KA]]
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[[Category: Transcription regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 4 09:59:59 2008''
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Current revision

Crystal Structure of the sensor domain of the transcriptional regulator DNR from Pseudomonas aeruginosa

PDB ID 2z69

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