1by3

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(New page: 200px<br /><applet load="1by3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1by3, resolution 2.74&Aring;" /> '''FHUA FROM E. COLI'''...)
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[[Image:1by3.jpg|left|200px]]<br /><applet load="1by3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1by3, resolution 2.74&Aring;" />
 
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'''FHUA FROM E. COLI'''<br />
 
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==Overview==
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==FHUA FROM E. COLI==
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FhuA protein facilitates ligand-gated transport of ferrichrome-bound iron, across Escherichia coli outer membranes. X-ray analysis at 2.7 A, resolution reveals two distinct conformations in the presence and absence, of ferrichrome. The monomeric protein consists of a hollow, 22-stranded, antiparallel beta barrel (residues 160-714), which is obstructed by a plug, (residues 19-159). The binding site of ferrichrome, an aromatic pocket, near the cell surface, undergoes minor changes upon association with the, ligand. These are propagated and amplified across the plug, eventually, resulting in substantially different protein conformations at the, periplasmic face. Our findings reveal the mechanism of signal transmission, and suggest how the energy-transducing TonB complex senses ligand binding.
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<StructureSection load='1by3' size='340' side='right'caption='[[1by3]], [[Resolution|resolution]] 2.74&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1by3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BY3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.74&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OES:N-OCTYL-2-HYDROXYETHYL+SULFOXIDE'>OES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1by3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1by3 OCA], [https://pdbe.org/1by3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1by3 RCSB], [https://www.ebi.ac.uk/pdbsum/1by3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1by3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FHUA_ECOLI FHUA_ECOLI] This receptor binds the ferrichrome-iron ligand. It interacts with the TonB protein, which is responsible for energy coupling of the ferrichrome-promoted iron transport system. Acts as a receptor for bacteriophage T5 as well as T1, phi80 and colicin M. Binding of T5 triggers the opening of a high conductance ion channel. Can also transport the antibiotic albomycin.<ref>PMID:8617231</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/1by3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1by3 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BY3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with OES as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BY3 OCA].
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*[[Ferric hydroxamate uptake receptor|Ferric hydroxamate uptake receptor]]
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== References ==
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==Reference==
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<references/>
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Transmembrane signaling across the ligand-gated FhuA receptor: crystal structures of free and ferrichrome-bound states reveal allosteric changes., Locher KP, Rees B, Koebnik R, Mitschler A, Moulinier L, Rosenbusch JP, Moras D, Cell. 1998 Dec 11;95(6):771-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9865695 9865695]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Koebnik, R.]]
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[[Category: Koebnik R]]
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[[Category: Locher, K.P.]]
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[[Category: Locher KP]]
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[[Category: Mitschler, A.]]
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[[Category: Mitschler A]]
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[[Category: Moras, D.]]
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[[Category: Moras D]]
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[[Category: Moulinier, L.]]
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[[Category: Moulinier L]]
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[[Category: Rees, B.]]
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[[Category: Rees B]]
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[[Category: Rosenbusch, J.P.]]
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[[Category: Rosenbusch JP]]
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[[Category: OES]]
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[[Category: fhua]]
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[[Category: iron transport]]
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[[Category: ligand-gated]]
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[[Category: membrane protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:00:59 2007''
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Current revision

FHUA FROM E. COLI

PDB ID 1by3

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