1byl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1byl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1byl, resolution 2.3&Aring;" /> '''BLEOMYCIN RESISTANCE ...)
Current revision (06:39, 7 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1byl.jpg|left|200px]]<br /><applet load="1byl" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1byl, resolution 2.3&Aring;" />
 
-
'''BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS'''<br />
 
-
==Overview==
+
==BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS==
-
The antibiotic bleomycin, a strong DNA cutting agent, is naturally, produced by actinomycetes which have developed a resistance mechanism, against such a lethal compound. The crystal structure, at 2.3 A, resolution, of a bleomycin resistance protein of 14 kDa reveals a, structure in two halves with the same alpha/beta fold despite no sequence, similarity. The crystal packing shows compact dimers with a hydrophobic, interface and involved in mutual chain exchange. Two independent solution, studies (analytical centrifugation and light scattering) showed that this, dimeric form is not a packing artefact but is indeed the functional one., Furthermore, light scattering also showed that one dimer binds two, antibiotic molecules as expected. A crevice located at the dimer, interface, as well as the results of a site-directed mutagenesis study, led to a model wherein two bleomycin molecules are completely sequestered, by one dimer. This provides a novel insight into antibiotic resistance due, to drug sequestering, and probably also into drug transport and excretion.
+
<StructureSection load='1byl' size='340' side='right'caption='[[1byl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1byl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptoalloteichus_hindustanus Streptoalloteichus hindustanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BYL FirstGlance]. <br>
-
1BYL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptoalloteichus_hindustanus Streptoalloteichus hindustanus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BYL OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1byl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1byl OCA], [https://pdbe.org/1byl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1byl RCSB], [https://www.ebi.ac.uk/pdbsum/1byl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1byl ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Crystal structure and site-directed mutagenesis of a bleomycin resistance protein and their significance for drug sequestering., Dumas P, Bergdoll M, Cagnon C, Masson JM, EMBO J. 1994 Jun 1;13(11):2483-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7516875 7516875]
+
== Function ==
-
[[Category: Single protein]]
+
[https://www.uniprot.org/uniprot/BLE_STRHI BLE_STRHI] Binding protein with a strong affinity to the bleomycin family of antibiotics.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/by/1byl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1byl ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Streptoalloteichus hindustanus]]
[[Category: Streptoalloteichus hindustanus]]
-
[[Category: Bergdoll, M.]]
+
[[Category: Bergdoll M]]
-
[[Category: Cagnon, C.]]
+
[[Category: Cagnon C]]
-
[[Category: Dumas, P.]]
+
[[Category: Dumas P]]
-
[[Category: Masson, J.M.]]
+
[[Category: Masson JM]]
-
[[Category: antibiotic resistance]]
+
-
[[Category: bleomycin]]
+
-
[[Category: chain swapping]]
+
-
[[Category: drug sequestering]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:01:49 2007''
+

Current revision

BLEOMYCIN RESISTANCE PROTEIN FROM STREPTOALLOTEICHUS HINDUSTANUS

PDB ID 1byl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1byl"
Personal tools