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1bz9

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(New page: 200px<br /><applet load="1bz9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bz9, resolution 2.8&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1bz9.gif|left|200px]]<br /><applet load="1bz9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bz9, resolution 2.8&Aring;" />
 
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'''CRYSTAL STRUCTURE OF MURINE CLASS I MHC H2-DB COMPLEXED WITH A SYNTHETIC PEPTIDE P1027'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF MURINE CLASS I MHC H2-DB COMPLEXED WITH A SYNTHETIC PEPTIDE P1027==
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The T cell receptor (TCR), from a xeno-reactive murine cytotoxic T, lymphocyte clone AHIII12.2, recognizes murine H-2Db complexed with peptide, p1027 (FAPGVFPYM), as well as human HLA-A2.1 complexed with peptide p1049, (ALWGFFPVL). A commonly proposed model (the molecular mimicry model) used, to explain TCR cross-reactivity suggests that the molecular surfaces of, the recognized complexes are similar in shape, charge, or both, in spite, of the primary sequence differences. To examine the mechanism of, xeno-reactivity of AHIII12.2, we have determined the crystal structures of, A2/p1049 and Db/p1027 to 2.5 A and 2.8 A resolution, respectively. The, crystal structures show that the TCR footprint regions of the two class I, complexes are significantly different in shape and charge. We propose that, rather than simple molecular mimicry, unpredictable arrays of common and, differential contacts on the two class I complexes are used for their, recognition by the same TCR.
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<StructureSection load='1bz9' size='340' side='right'caption='[[1bz9]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bz9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1a9d 1a9d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BZ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BZ9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bz9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bz9 OCA], [https://pdbe.org/1bz9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bz9 RCSB], [https://www.ebi.ac.uk/pdbsum/1bz9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bz9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HA11_MOUSE HA11_MOUSE] Involved in the presentation of foreign antigens to the immune system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bz/1bz9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bz9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The T cell receptor (TCR), from a xeno-reactive murine cytotoxic T lymphocyte clone AHIII12.2, recognizes murine H-2Db complexed with peptide p1027 (FAPGVFPYM), as well as human HLA-A2.1 complexed with peptide p1049 (ALWGFFPVL). A commonly proposed model (the molecular mimicry model) used to explain TCR cross-reactivity suggests that the molecular surfaces of the recognized complexes are similar in shape, charge, or both, in spite of the primary sequence differences. To examine the mechanism of xeno-reactivity of AHIII12.2, we have determined the crystal structures of A2/p1049 and Db/p1027 to 2.5 A and 2.8 A resolution, respectively. The crystal structures show that the TCR footprint regions of the two class I complexes are significantly different in shape and charge. We propose that rather than simple molecular mimicry, unpredictable arrays of common and differential contacts on the two class I complexes are used for their recognition by the same TCR.
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==About this Structure==
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Structural evidence of T cell xeno-reactivity in the absence of molecular mimicry.,Zhao R, Loftus DJ, Appella E, Collins EJ J Exp Med. 1999 Jan 18;189(2):359-70. PMID:9892618<ref>PMID:9892618</ref>
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1BZ9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure superseeds the now removed PDB entry 1A9D. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BZ9 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural evidence of T cell xeno-reactivity in the absence of molecular mimicry., Zhao R, Loftus DJ, Appella E, Collins EJ, J Exp Med. 1999 Jan 18;189(2):359-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9892618 9892618]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 1bz9" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Collins, E.J.]]
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[[Category: Zhao, R.]]
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[[Category: murine class i mhc/peptide complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:02:26 2007''
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==See Also==
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*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
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*[[MHC 3D structures|MHC 3D structures]]
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*[[MHC I 3D structures|MHC I 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Collins EJ]]
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[[Category: Zhao R]]

Current revision

CRYSTAL STRUCTURE OF MURINE CLASS I MHC H2-DB COMPLEXED WITH A SYNTHETIC PEPTIDE P1027

PDB ID 1bz9

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