1c0a

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(New page: 200px<br /><applet load="1c0a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c0a, resolution 2.40&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1c0a.gif|left|200px]]<br /><applet load="1c0a" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1c0a, resolution 2.40&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE E. COLI ASPARTYL-TRNA SYNTHETASE : TRNAASP : ASPARTYL-ADENYLATE COMPLEX'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE E. COLI ASPARTYL-TRNA SYNTHETASE : TRNAASP : ASPARTYL-ADENYLATE COMPLEX==
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The 2.4 A crystal structure of the Escherichia coli aspartyl-tRNA, synthetase (AspRS)-tRNA(Asp)-aspartyl-adenylate complex shows the two, substrates poised for the transfer of the aspartic acid moiety from the, adenylate to the 3'-hydroxyl of the terminal adenosine of the tRNA. A, general molecular mechanism is proposed for the second step of the, aspartylation reaction that accounts for the observed conformational, changes, notably in the active site pocket. The stabilization of the, transition state is mediated essentially by two amino acids: the class II, invariant arginine of motif 2 and the eubacterial-specific Gln231, which, in eukaryotes and archaea is replaced by a structurally non-homologous, serine. Two archetypal RNA-protein modes of interactions are observed: the, anticodon stem-loop, including the wobble base Q, binds to the N-terminal, beta-barrel domain through direct protein-RNA interactions, while the, binding of the acceptor stem involves both direct and water-mediated, hydrogen bonds in an original recognition scheme.
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<StructureSection load='1c0a' size='340' side='right'caption='[[1c0a]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1c0a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C0A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4SU:4-THIOURIDINE-5-MONOPHOSPHATE'>4SU</scene>, <scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=AMO:ASPARTYL-ADENOSINE-5-MONOPHOSPHATE'>AMO</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=QUO:2-AMINO-7-DEAZA-(2,3-DIHYDROXY-CYCLOPENTYLAMINO)-GUANOSINE-5-MONOPHOSPHATE'>QUO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c0a OCA], [https://pdbe.org/1c0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c0a RCSB], [https://www.ebi.ac.uk/pdbsum/1c0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c0a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYD_ECOLI SYD_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c0/1c0a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c0a ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1C0A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, AMP and AMO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aspartate--tRNA_ligase Aspartate--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.12 6.1.1.12] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C0A OCA].
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
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==Reference==
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__TOC__
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Synthesis of aspartyl-tRNA(Asp) in Escherichia coli--a snapshot of the second step., Eiler S, Dock-Bregeon A, Moulinier L, Thierry JC, Moras D, EMBO J. 1999 Nov 15;18(22):6532-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10562565 10562565]
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</StructureSection>
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[[Category: Aspartate--tRNA ligase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Dock-Bregeon, A.C.]]
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[[Category: Dock-Bregeon A-C]]
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[[Category: Eiler, S.]]
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[[Category: Eiler S]]
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[[Category: Moras, D.]]
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[[Category: Moras D]]
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[[Category: Moulinier, L.]]
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[[Category: Moulinier L]]
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[[Category: Thierry, J.C.]]
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[[Category: Thierry J-C]]
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[[Category: AMO]]
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[[Category: AMP]]
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[[Category: SO4]]
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[[Category: protein-rna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:03:32 2007''
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Current revision

CRYSTAL STRUCTURE OF THE E. COLI ASPARTYL-TRNA SYNTHETASE : TRNAASP : ASPARTYL-ADENYLATE COMPLEX

PDB ID 1c0a

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