2k33
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2k33 is ON HOLD until Paper Publication Authors: Slynko, V., Schubert, M., Numao, S., Kowarik, M., Aebi, M., Allain, F.H.-T. Description: Solution ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of an N-glycosylated protein using in vitro glycosylation== | |
| + | <StructureSection load='2k33' size='340' side='right'caption='[[2k33]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2k33]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K33 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=B6D:2,4-BIS(ACETYLAMINO)-2,4,6-TRIDEOXY-BETA-D-GLUCOPYRANOSE'>B6D</scene>, <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k33 OCA], [https://pdbe.org/2k33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k33 RCSB], [https://www.ebi.ac.uk/pdbsum/2k33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k33 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8RTE5_CAMJU Q8RTE5_CAMJU] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/2k33_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k33 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Although there is great interest in three-dimensional structures of glycoproteins and complex oligosaccharides, their structural determination have been hampered by inhomogeneous and incomplete glycosylation, poor expression, low tendency to crystallize, and severe chemical shift overlap. Using segmental labeling of the glycan and the protein component by in vitro glycosylation, we developed a novel method of NMR structural determination that overcomes some of these problems. Highly homogeneously glycosylated proteins in milligram amounts can be obtained. This allowed the determination of the structure of an N-linked glycoprotein from Campylobacter jejuni. The glycosylation acceptor site was found to be in a flexible loop. The presented methodology extends the observable NOE distance limit of oligosaccharides significantly over 4 A, resulting in a high number of distance restraints per glycosidic linkage. A well-defined glycan structure was obtained. | ||
| - | + | NMR structure determination of a segmentally labeled glycoprotein using in vitro glycosylation.,Slynko V, Schubert M, Numao S, Kowarik M, Aebi M, Allain FH J Am Chem Soc. 2009 Jan 28;131(3):1274-81. PMID:19154179<ref>PMID:19154179</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2k33" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Campylobacter jejuni]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Aebi M]] | ||
| + | [[Category: Allain FH-T]] | ||
| + | [[Category: Kowarik M]] | ||
| + | [[Category: Numao S]] | ||
| + | [[Category: Schubert M]] | ||
| + | [[Category: Slynko V]] | ||
Current revision
Solution structure of an N-glycosylated protein using in vitro glycosylation
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