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2ro2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2ro2 is ON HOLD until Paper Publication Authors: Gallego, J., Dufour, D., Gago, S., De la Pena, M., Flores, R. Description: Solution structure of d...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme== | |
| + | <StructureSection load='2ro2' size='340' side='right'caption='[[2ro2]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2ro2]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RO2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ro2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ro2 OCA], [https://pdbe.org/2ro2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ro2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ro2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ro2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Loop-loop tertiary interactions play a key role in the folding and catalytic activity of natural hammerhead ribozymes. Using a combination of NMR spectroscopy, site-directed mutagenesis and kinetic and infectivity analyses, we have examined the structure and function of loops 1 and 2 of the (+) and (-) hammerheads of chrysanthemum chlorotic mottle viroid RNA. In both hammerheads, loop 1 is a heptanucleotide hairpin loop containing an exposed U at its 5' side and an extrahelical U at its 3'-side critical for the catalytic activity of the ribozyme in vitro and for viroid infectivity in vivo, whereas loop 2 has a key opened A at its 3'-side. These structural features promote a specific loop-loop interaction motif across the major groove. The essential features of this tertiary structure element, base pairing between the 5' U of loop 1 and the 3' A of loop 2, and interaction of the extrahelical pyrimidine of loop 1 with loop 2, are likely shared by a significant fraction of natural hammerheads. | ||
| - | + | Structure-function analysis of the ribozymes of chrysanthemum chlorotic mottle viroid: a loop-loop interaction motif conserved in most natural hammerheads.,Dufour D, de la Pena M, Gago S, Flores R, Gallego J Nucleic Acids Res. 2008 Nov 29. PMID:19043070<ref>PMID:19043070</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2ro2" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| - | + | *[[Ribozyme 3D structures|Ribozyme 3D structures]] | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Dufour D]] | ||
| + | [[Category: Flores R]] | ||
| + | [[Category: Gago S]] | ||
| + | [[Category: Gallego J]] | ||
| + | [[Category: De la Pena M]] | ||
Current revision
Solution structure of domain I of the negative polarity CChMVd hammerhead ribozyme
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