2zkz
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 2zkz is ON HOLD until Paper Publication Authors: Zhao, H., Volkov, A., Veldore, V.H., Varughese, K.I. Description: Crystal structure of the transcr...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the transcriptional repressor PagR of Bacillus anthracis== | |
| + | <StructureSection load='2zkz' size='340' side='right'caption='[[2zkz]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2zkz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZKZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZKZ FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pagR, tcrA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zkz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zkz OCA], [https://pdbe.org/2zkz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zkz RCSB], [https://www.ebi.ac.uk/pdbsum/2zkz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zkz ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[https://www.uniprot.org/uniprot/PAGR_BACAN PAGR_BACAN]] Represses the expression of the pagA and atxA genes. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zk/2zkz_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zkz ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | PagR is a transcriptional repressor in Bacillus anthracis that controls the chromosomal S-layer genes eag and sap, and downregulates the protective antigen pagA gene by direct binding to their promoter regions. The PagR protein sequence is similar to those of members of the ArsR repressor family involved in the repression of arsenate-resistance genes in numerous bacteria. The crystal structure of PagR was solved using multi-wavelength anomalous diffraction (MAD) techniques and was refined with 1.8 A resolution diffraction data. The PagR molecules form dimers, as observed in all SmtB/ArsR repressor family proteins. In the crystal lattice four PagR dimers pack together to form an inactive octamer. Model-building studies suggest that the dimer binds to a DNA duplex with a bend of around 4 degrees. | ||
| - | + | Crystal structure of the transcriptional repressor PagR of Bacillus anthracis.,Zhao H, Volkov A, Veldore VH, Hoch JA, Varughese KI Microbiology. 2010 Feb;156(Pt 2):385-91. Epub 2009 Nov 19. PMID:19926656<ref>PMID:19926656</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2zkz" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Varughese, K I]] | ||
| + | [[Category: Veldore, V H]] | ||
| + | [[Category: Volkov, A]] | ||
| + | [[Category: Zhao, H]] | ||
| + | [[Category: Dimer]] | ||
| + | [[Category: Dna-binding]] | ||
| + | [[Category: Hth motif]] | ||
| + | [[Category: Protein-dna]] | ||
| + | [[Category: Transcription]] | ||
| + | [[Category: Transcription regulation]] | ||
| + | [[Category: Transcriptional repressor]] | ||
Current revision
Crystal structure of the transcriptional repressor PagR of Bacillus anthracis
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