2r60

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[[Image:2r60.jpg|left|200px]]
 
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==Structure of apo Sucrose Phosphate Synthase (SPS) of Halothermothrix orenii==
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The line below this paragraph, containing "STRUCTURE_2r60", creates the "Structure Box" on the page.
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<StructureSection load='2r60' size='340' side='right'caption='[[2r60]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2r60]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothermothrix_orenii Halothermothrix orenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R60 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r60 OCA], [https://pdbe.org/2r60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r60 RCSB], [https://www.ebi.ac.uk/pdbsum/2r60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r60 ProSAT]</span></td></tr>
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{{STRUCTURE_2r60| PDB=2r60 | SCENE= }}
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</table>
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== Function ==
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'''Structure of apo Sucrose Phosphate Synthase (SPS) of Halothermothrix orenii'''
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[https://www.uniprot.org/uniprot/B2CCB8_9FIRM B2CCB8_9FIRM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Sucrose phosphate synthase (SPS) catalyzes the transfer of a glycosyl group from an activated donor sugar, such as uridine diphosphate glucose (UDP-Glc), to a saccharide acceptor d-fructose 6-phosphate (F6P), resulting in the formation of UDP and d-sucrose-6'-phosphate (S6P). This is a central regulatory process in the production of sucrose in plants, cyanobacteria, and proteobacteria. Here, we report the crystal structure of SPS from the nonphotosynthetic bacterium Halothermothrix orenii and its complexes with the substrate F6P and the product S6P. SPS has two distinct Rossmann-fold domains with a large substrate binding cleft at the interdomain interface. Structures of two complexes show that both the substrate F6P and the product S6P bind to the A-domain of SPS. Based on comparative analysis of the SPS structure with other related enzymes, the donor substrate, nucleotide diphosphate glucose, binds to the B-domain of SPS. Furthermore, we propose a mechanism of catalysis by H. orenii SPS. Our findings indicate that SPS from H. orenii may represent a valid model for the catalytic domain of plant SPSs and thus may provide useful insight into the reaction mechanism of the plant enzyme.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r6/2r60_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2R60 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halothermothrix_orenii Halothermothrix orenii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R60 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r60 ConSurf].
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The Structure of Sucrose Phosphate Synthase from Halothermothrix orenii Reveals Its Mechanism of Action and Binding Mode., Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J, Plant Cell. 2008 Apr;20(4):1059-72. Epub 2008 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18424616 18424616]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Halothermothrix orenii]]
[[Category: Halothermothrix orenii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Sucrose-phosphate synthase]]
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[[Category: Chua TK]]
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[[Category: Chua, T K.]]
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[[Category: Sivaraman J]]
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[[Category: Sivaraman, J.]]
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[[Category: Rossmann-fold]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:44:21 2008''
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Current revision

Structure of apo Sucrose Phosphate Synthase (SPS) of Halothermothrix orenii

PDB ID 2r60

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