1c8s

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(New page: 200px<br /><applet load="1c8s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c8s, resolution 2.00&Aring;" /> '''BACTERIORHODOPSIN D9...)
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[[Image:1c8s.gif|left|200px]]<br /><applet load="1c8s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1c8s, resolution 2.00&Aring;" />
 
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'''BACTERIORHODOPSIN D96N LATE M STATE INTERMEDIATE'''<br />
 
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==Overview==
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==BACTERIORHODOPSIN D96N LATE M STATE INTERMEDIATE==
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Crystal structures of the Asp96 to Asn mutant of the light-driven proton, pump bacteriorhodopsin and its M photointermediate produced by, illumination at ambient temperature have been determined to 1.8 and 2.0, angstroms resolution, respectively. The trapped photoproduct corresponds, to the late M state in the transport cycle-that is, after proton transfer, to Asp85 and release of a proton to the extracellular membrane surface, but before reprotonation of the deprotonated retinal Schiff base. Its, density map describes displacements of side chains near the retinal, induced by its photoisomerization to 13-cis,15-anti and an extensive, rearrangement of the three-dimensional network of hydrogen-bonded residues, and bound water that accounts for the changed pKa values (where Ka is the, acid constant) of the Schiff base and Asp85. The structural changes, detected suggest the means for conserving energy at the active site and, for ensuring the directionality of proton translocation.
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<StructureSection load='1c8s' size='340' side='right'caption='[[1c8s]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1c8s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C8S FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene>, <scene name='pdbligand=SQU:2,10,23-TRIMETHYL-TETRACOSANE'>SQU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c8s OCA], [https://pdbe.org/1c8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c8s RCSB], [https://www.ebi.ac.uk/pdbsum/1c8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c8s ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c8/1c8s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c8s ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Crystal structures of the Asp96 to Asn mutant of the light-driven proton pump bacteriorhodopsin and its M photointermediate produced by illumination at ambient temperature have been determined to 1.8 and 2.0 angstroms resolution, respectively. The trapped photoproduct corresponds to the late M state in the transport cycle-that is, after proton transfer to Asp85 and release of a proton to the extracellular membrane surface, but before reprotonation of the deprotonated retinal Schiff base. Its density map describes displacements of side chains near the retinal induced by its photoisomerization to 13-cis,15-anti and an extensive rearrangement of the three-dimensional network of hydrogen-bonded residues and bound water that accounts for the changed pKa values (where Ka is the acid constant) of the Schiff base and Asp85. The structural changes detected suggest the means for conserving energy at the active site and for ensuring the directionality of proton translocation.
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==About this Structure==
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Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution.,Luecke H, Schobert B, Richter HT, Cartailler JP, Lanyi JK Science. 1999 Oct 8;286(5438):255-61. PMID:10514362<ref>PMID:10514362</ref>
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1C8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum] with LI1, SQU and RET as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C8S OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution., Luecke H, Schobert B, Richter HT, Cartailler JP, Lanyi JK, Science. 1999 Oct 8;286(5438):255-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10514362 10514362]
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</div>
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[[Category: Halobacterium salinarum]]
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<div class="pdbe-citations 1c8s" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Luecke, H.]]
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[[Category: LI1]]
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[[Category: RET]]
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[[Category: SQU]]
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[[Category: d96n m intermediate]]
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[[Category: haloarchaea]]
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[[Category: ion pump]]
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[[Category: ion transport]]
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[[Category: lipids]]
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[[Category: membrane protein]]
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[[Category: merohedral twinning]]
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[[Category: photoreceptor]]
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[[Category: retinal protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:15:52 2007''
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==See Also==
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*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Halobacterium salinarum]]
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[[Category: Large Structures]]
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[[Category: Luecke H]]

Current revision

BACTERIORHODOPSIN D96N LATE M STATE INTERMEDIATE

PDB ID 1c8s

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