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1cbr

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(New page: 200px<br /><applet load="1cbr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cbr, resolution 2.9&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1cbr.jpg|left|200px]]<br /><applet load="1cbr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1cbr, resolution 2.9&Aring;" />
 
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'''CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID==
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BACKGROUND: Retinoic acid (RA) plays a fundamental role in diverse, cellular activities. Cellular RA binding proteins (CRABPs) are thought to, act by modulating the amount of RA available to nuclear RA receptors., CRABPs and cellular retinol-binding proteins (CRBPs) share a unique fold, of two orthogonal beta-sheets that encapsulate their ligands. It has been, suggested that a trio of residues are the prime determinants defining the, high specificity of CRBPs and CRABPs for their physiological ligands., RESULTS: Bovine/murine CRABP I and human CRABP II have been crystallized, in complex with their natural ligand, all-trans-RA. Human CRABP II has, also been crystallized in complex with a synthetic retinoid, 'compound, 19'. Their structures have been determined and refined at resolutions of, 2.9 A, 1.8 A and 2.2 A, respectively. CONCLUSIONS: The retinoid-binding, site in CRABPs differs significantly from that observed in CRBP., Structural changes in three juxtaposed areas of the protein create a new, displaced binding site for RA. The carboxylate of the ligand interacts, with the expected trio of residues (Arg132, Tyr134 and Arg111; CRABP II, numbering). The RA ligand is almost flat with the beta-ionone ring showing, a significant deviation (-33 degrees) from a cis conformation relative to, the isoprene tail. The edge atoms of the beta-ionone ring are accessible, to solvent in a suitable orientation for presentation to metabolizing, enzymes. The bulkier synthetic retinoid causes small conformational, changes in the protein structure.
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<StructureSection load='1cbr' size='340' side='right'caption='[[1cbr]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1cbr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=REA:RETINOIC+ACID'>REA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbr OCA], [https://pdbe.org/1cbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbr RCSB], [https://www.ebi.ac.uk/pdbsum/1cbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RABP1_MOUSE RABP1_MOUSE] Cytosolic CRABPs may regulate the access of retinoic acid to the nuclear retinoic acid receptors.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1CBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with REA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CBR OCA].
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*[[CRABP I ( Cellular Retinoic Acid Binding Protein )|CRABP I ( Cellular Retinoic Acid Binding Protein )]]
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*[[Cellular retinoic acid-binding protein 3D structures|Cellular retinoic acid-binding protein 3D structures]]
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==Reference==
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*[[Gustavo Elberto Epalza Sanchez/Sandbox 1|Gustavo Elberto Epalza Sanchez/Sandbox 1]]
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Crystal structures of cellular retinoic acid binding proteins I and II in complex with all-trans-retinoic acid and a synthetic retinoid., Kleywegt GJ, Bergfors T, Senn H, Le Motte P, Gsell B, Shudo K, Jones TA, Structure. 1994 Dec 15;2(12):1241-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7704533 7704533]
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*[[Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)|Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)]]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Bergfors T]]
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[[Category: Bergfors, T.]]
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[[Category: Jones TA]]
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[[Category: Jones, T.A.]]
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[[Category: Kleywegt GJ]]
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[[Category: Kleywegt, G.J.]]
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[[Category: REA]]
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[[Category: retinoic-acid transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:19:36 2007''
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Current revision

CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID

PDB ID 1cbr

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